Effect of oxygen concentrations on sodium iodide symporter expression and iodide uptake and hCG expression in human choriocarcinoma BeWo cells

Li, Huika, Landers, Kelly, Patel, Jatin, Richard, Kerry and Mortimer, Robin H. (2011) Effect of oxygen concentrations on sodium iodide symporter expression and iodide uptake and hCG expression in human choriocarcinoma BeWo cells. American Journal of Physiology: Endocrinology and Metabolism, 300 6: E1085-E1091. doi:10.1152/ajpendo.00679.2010


Author Li, Huika
Landers, Kelly
Patel, Jatin
Richard, Kerry
Mortimer, Robin H.
Title Effect of oxygen concentrations on sodium iodide symporter expression and iodide uptake and hCG expression in human choriocarcinoma BeWo cells
Journal name American Journal of Physiology: Endocrinology and Metabolism   Check publisher's open access policy
ISSN 0193-1849
1522-1555
Publication date 2011-06-01
Sub-type Article (original research)
DOI 10.1152/ajpendo.00679.2010
Volume 300
Issue 6
Start page E1085
End page E1091
Total pages 7
Place of publication Bethesda, MD, United States
Publisher American Physiological Society
Language eng
Formatted abstract
Normal human fetal development requires an adequate supply of thyroid hormone from conception. Until about 16 wk gestation this is supplied entirely by placental transfer of maternal hormone. Subsequently, the fetal thyroid synthesizes thyroid hormones, requiring a supply of maternal iodide. Trophoblast iodide transfer is mediated by the apical sodium iodide symporter (NIS). Placental oxygen levels are low in early pregnancy (∼1%), rising with placental vascularisation to a plateau of ∼8% at about 16 wk. Although the impact of these changing oxygen levels on placental implantation is well recognized, effects on trophoblast materno-fetal exchange are less understood. We investigated expression of the NIS regulator hCG, NIS mRNA expression, and I125 uptake in choriocarcinoma BeWo cells (a model of the trophoblast) cultured in 1 and 8% oxygen and in room air (21% oxygen). Expression of NIS and hCG mRNA and protein was low at 1% oxygen but rose significantly at 8 and at 21%. This was reflected in significant increases in I125 uptake. Desferrioxamine, an iron chelator and hypoxia mimic, decreased NIS and hCG expression and I125 uptake in BeWo cells. NIS expression and I125 uptake in cells grown at 1% oxygen were not increased by addition of hCG (2,500 IU/l). We infer that placental NIS mRNA and protein expression are regulated by oxygen, rising with vascularization of the placenta in the late first trimester, a time when fetal iodide requirements are increasing.
Keyword Human chorionic gonadotropin
Placenta
Hypoxia
Thyroid hormone
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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