Self-adjuvanting modular virus-like particles for mucosal vaccination against group A streptococcus (GAS)

Rivera-Hernandez, Tania, Hartas, Jon, Wu, Yang, Chuan, Yap P., Lua, Linda H. L., Good, Michael, Batzloff, Michael R. and Middelberg, Anton P. J. (2013) Self-adjuvanting modular virus-like particles for mucosal vaccination against group A streptococcus (GAS). Vaccine, 31 15: 1950-1955. doi:10.1016/j.vaccine.2013.02.013

Author Rivera-Hernandez, Tania
Hartas, Jon
Wu, Yang
Chuan, Yap P.
Lua, Linda H. L.
Good, Michael
Batzloff, Michael R.
Middelberg, Anton P. J.
Title Self-adjuvanting modular virus-like particles for mucosal vaccination against group A streptococcus (GAS)
Journal name Vaccine   Check publisher's open access policy
ISSN 0264-410X
Publication date 2013-04
Sub-type Article (original research)
DOI 10.1016/j.vaccine.2013.02.013
Volume 31
Issue 15
Start page 1950
End page 1955
Total pages 6
Place of publication London, United Kingdom
Publisher Elsevier
Collection year 2014
Language eng
Abstract Group A streptococcus (GAS) causes a wide range of diseases, some of them related to autoimmune diseases triggered by repeated GAS infections. Despite the fact that GAS primarily colonizes the mucosal epithelium of the pharynx, the main mechanism of action of most vaccine candidates is based on development of systemic antibodies that do not cross-react with host tissues, neglecting the induction of mucosal immunity that could potentially block disease transmission. Peptide antigens from GAS M-surface protein can confer protection against infection; however, translation of such peptides into immunogenic mucosal vaccines that can be easily manufactured remains a challenge. In this work, a modular murine polyomavirus (MuPyV) virus-like particle (VLP) was engineered to display a GAS antigenic peptide, J8i. Heterologous modules containing one or two J8i antigen elements were integrated with the MuPyV VLP, and produced using microbial protein expression, standard purification techniques and in vitro VLP assembly. Both modular VLPs, when delivered intranasally to outbred mice without adjuvant, induced significant titers of J8i-specific IgG and IgA antibodies, indicating significant systemic and mucosal responses, respectively. GAS colonization in the throats of mice challenged intranasally was reduced in these immunized mice, and protection against lethal challenge was observed. This study shows that modular MuPyV VLPs prepared using microbial synthesis have potential to facilitate cost-effective vaccine delivery to remote communities through the use of mucosal immunization.
Keyword Virus-like particles
Group A streptococcus
Intranasal vaccine
Intranasal challenge
Bacterial colonization
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
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