From deep sequencing to viral tagging: recent advances in viral metagenomics

Willner, Dana and Hugenholtz, Philip (2013) From deep sequencing to viral tagging: recent advances in viral metagenomics. Bioessays, 35 5: 436-442. doi:10.1002/bies.201200174

Author Willner, Dana
Hugenholtz, Philip
Title From deep sequencing to viral tagging: recent advances in viral metagenomics
Journal name Bioessays   Check publisher's open access policy
ISSN 0265-9247
Publication date 2013-05
Year available 2013
Sub-type Article (original research)
DOI 10.1002/bies.201200174
Volume 35
Issue 5
Start page 436
End page 442
Total pages 7
Place of publication Chichester, West Sussex, United Kingdom
Publisher John Wiley & Sons
Collection year 2014
Language eng
Abstract Culture-independent high-throughput sequencing has provided unprecedented insights into microbial ecology, particularly for Earth’s most ubiquitous and diverse inhabitants – the viruses. A plethora of methods now exist for amplifying the vanishingly small amounts of nucleic acids in natural viral communities in order to sequence them, and sequencing depth is now so great that viral genomes can be detected and assembled even amid large concentrations of non-viral DNA. Complementing these advances in amplification and sequencing is the ability to physically link fluorescently labeled viruses to their host cells via high-throughput flow sorting. Sequencing of such isolated virus–host pairs facilitates cultivation-independent exploration of the natural host range of viruses. Within the next decade, as these technologies become widespread, we can expect to see a systematic expansion of our knowledge of viruses and their hosts.
Keyword Bioinformatics
Viral metagenomics
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

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