A sleep/wake circuit controls isoflurane sensitivity in Drosophila

Kottler, Benjamin, Bao, Hong, Zalucki, Oressia, Imlach, Wendy, Troup, Michael, van Alphen, Bart, Paulk, Angelique, Zhang, Bing and van Swinderen, Bruno (2013) A sleep/wake circuit controls isoflurane sensitivity in Drosophila. Current Biology, 23 7: 594-598. doi:10.1016/j.cub.2013.02.021

Author Kottler, Benjamin
Bao, Hong
Zalucki, Oressia
Imlach, Wendy
Troup, Michael
van Alphen, Bart
Paulk, Angelique
Zhang, Bing
van Swinderen, Bruno
Title A sleep/wake circuit controls isoflurane sensitivity in Drosophila
Formatted title
A sleep/wake circuit controls isoflurane sensitivity in Drosophila
Journal name Current Biology   Check publisher's open access policy
ISSN 0960-9822
Publication date 2013-04
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.cub.2013.02.021
Volume 23
Issue 7
Start page 594
End page 598
Total pages 5
Place of publication Cambridge, MA, United States
Publisher Cell Press
Collection year 2014
Language eng
Formatted abstract
General anesthesia remains a mysterious phenomenon, even though a number of compelling target proteins and processes have been proposed [1]. General anesthetics such as isoflurane abolish behavioral responsiveness in all animals, and in the mammalian brain, these diverse compounds probably achieve this in part by targeting endogenous sleep mechanisms [2, 3]. However, most animals sleep [4], and they are therefore likely to have conserved sleep processes. A decade of neurogenetic studies of arousal in Drosophila melanogaster have identified a number of different neurons and brain structures that modulate sleep duration in the fly brain [5-9], but it has remained unclear until recently whether any neurons might form part of a dedicated circuit that actively controls sleep and wake states in the fly brain, as has been proposed for the mammalian brain [10]. We studied general anesthesia in Drosophila by measuring stimulus-induced locomotion under isoflurane gas exposure. Using a syntaxin1A gain-of-function construct, we found that increasing synaptic activity in different Drosophila neurons could produce hypersensitivity or resistance to isoflurane. We uncover a common pathway in the fly brain controlling both sleep duration and isoflurane sensitivity, centered on monoaminergic modulation of sleep-promoting neurons of the fan-shaped body.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Brain Institute Publications
Official 2014 Collection
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Citation counts: TR Web of Science Citation Count  Cited 16 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 18 times in Scopus Article | Citations
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