Comparison of the cobas 4800 CT/NG test with culture for detecting neisseria gonorrhoeae in genital and nongenital specimens in a low-prevalence population in New Zealand

Bromhead, Collette, Miller, Amanda, Jones, Mark and Whiley, David (2013) Comparison of the cobas 4800 CT/NG test with culture for detecting neisseria gonorrhoeae in genital and nongenital specimens in a low-prevalence population in New Zealand. Journal of Clinical Microbiology, 51 5: 1505-1509. doi:10.1128/JCM.03223-12

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Author Bromhead, Collette
Miller, Amanda
Jones, Mark
Whiley, David
Title Comparison of the cobas 4800 CT/NG test with culture for detecting neisseria gonorrhoeae in genital and nongenital specimens in a low-prevalence population in New Zealand
Formatted title
Comparison of the cobas 4800 CT/NG test with culture for detecting neisseria gonorrhoeae in genital and nongenital specimens in a low-prevalence population in New Zealand
Journal name Journal of Clinical Microbiology   Check publisher's open access policy
ISSN 0095-1137
1098-660X
Publication date 2013-05
Year available 2013
Sub-type Article (original research)
DOI 10.1128/JCM.03223-12
Open Access Status File (Publisher version)
Volume 51
Issue 5
Start page 1505
End page 1509
Total pages 5
Place of publication Washington, United States
Publisher American Society for Microbiology
Collection year 2014
Language eng
Formatted abstract
To assess the clinical utility of replacing microbial culture for Neisseria gonorrhoeae with a nucleic acid amplification test (NAAT), we compared N. gonorrhoeae culture with the cobas 4800 CT/NG test for 18,247 urogenital and 666 nongenital samples. For urogenital specimens, the sensitivity, specificity, and positive and negative predictive values of the cobas N. gonorrhoeae PCR were 98.7%, 100%, 95.6%, and 100%, respectively, and for nongenital specimens, the values were 100%, 99.8%, 92.9%, and 100%, respectively. In our test population, 37% (10,185) of patients tested over the study period were screened for C. trachomatis by PCR but were not screened for gonorrhea by culture. Of these, 43 were N. gonorrhoeae positive by PCR and therefore went undiagnosed. The cobas 4800 CT/NG test diagnosed 33% (n = 30) more urogenital and 25% (n = 3) more rectal gonorrhea infections than culture and, based on the above performance indicators, does not require supplementary testing for urogenital or rectal specimens. The ability to test noninvasive specimens (such as urine and self-taken vulvovaginal swabs) for N. gonorrhoeae will enable more patients to be screened for infection, thus offering significant positive public health benefits.
Keyword Acid amplification tests
Chlamydia trachomatis
Susceptibility
Infections
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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