Novel PCR assay for determining the genetic sex of mice

McFarlane, L., Truong, V., Palmer, J. S. and Wilhelm, D. (2013) Novel PCR assay for determining the genetic sex of mice. Sexual Development, 7 4: 207-211. doi:10.1159/000348677


Author McFarlane, L.
Truong, V.
Palmer, J. S.
Wilhelm, D.
Title Novel PCR assay for determining the genetic sex of mice
Journal name Sexual Development   Check publisher's open access policy
ISSN 1661-5425
1661-5433
Publication date 2013-01
Sub-type Article (original research)
DOI 10.1159/000348677
Volume 7
Issue 4
Start page 207
End page 211
Total pages 5
Place of publication Basel, Switzerland
Publisher S. Karger AG
Collection year 2014
Language eng
Abstract A number of studies require the determination of the genetic sex of mouse embryos before sexual differentiation and/or of mutant mice that display partial or complete sex reversal. The majority of current methods for sexing by PCR involve multiplexing of 2 primer pairs. We have developed a novel sexing PCR using a single primer pair that amplifies fragments from the X and the Y chromosome with a clear size difference between the respective amplicons. This assay provides a rapid and reliable method to identify the genetic sex of mice across different mouse strains.
Keyword Genetic sex
Mouse
PCR
Sex determination
X chromosome
Y chromosome
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
Institute for Molecular Bioscience - Publications
 
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