Polymerase chain reaction-based screening for the ceftriaxone-resistant Neisseria gonorrhoeae F89 strain

Goire, N., Lahra, M. M., Ohnishi, M., Hogan, T., Liminios, A. E., Nissen, M. D., Sloots, T. P. and Whiley, D. M. (2013) Polymerase chain reaction-based screening for the ceftriaxone-resistant Neisseria gonorrhoeae F89 strain. Eurosurveillance, 18 14: 18-23. doi:10.2807/1560-7917.ES2013.18.14.20444


Author Goire, N.
Lahra, M. M.
Ohnishi, M.
Hogan, T.
Liminios, A. E.
Nissen, M. D.
Sloots, T. P.
Whiley, D. M.
Title Polymerase chain reaction-based screening for the ceftriaxone-resistant Neisseria gonorrhoeae F89 strain
Formatted title
Polymerase chain reaction-based screening for the ceftriaxone-resistant Neisseria gonorrhoeae F89 strain
Journal name Eurosurveillance   Check publisher's open access policy
ISSN 1025-496X
1560-7917
Publication date 2013-04
Sub-type Article (original research)
DOI 10.2807/1560-7917.ES2013.18.14.20444
Open Access Status DOI
Volume 18
Issue 14
Start page 18
End page 23
Total pages 6
Place of publication France
Publisher Centre Europeen pour la Surveillance Epidemiologique du SIDA
Collection year 2014
Language eng
Formatted abstract
Emergence and spread of Neisseria gonorrhoeae resistant to extended spectrum cephalosporins is a major problem threatening treatment of gonorrhoea and is further highlighted by the recent report of a second ceftriaxone-resistant N. gonorrhoeae strain (F89) in Europe, initially observed in France and subsequently identified in Spain. N. gonorrhoeae antimicrobial resistance (AMR) surveillance has acquired new importance and molecular tools have the potential to enhance bacterial culture-based methods. In this study, we established a polymerase chain reaction (PCR) protocol for direct detection of the F89 strain. A key component of this screening protocol was the development of a hybridisation probe-based melting curve analysis assay (mosaic501-hybPCR) to detect the presence of an A501P substitution on the N. gonorrhoeae mosaic penicillin binding protein 2 (PBP2) sequence, an important characteristic of the F89 strain. The mosaic501-hybPCR was evaluated using plasmid-derived positive controls (n=3) and characterised gonococcal (n=33) and non-gonococcal (n=58) isolates. The protocol was then applied to 159 clinical specimens from Sydney, Australia, collected during the first half of the year 2012 that were N. gonorrhoeae PCR-positive. Overall, the results indicate that the PCR-based protocol is suitable for direct detection of the N. gonorrhoeae F89 strain in non-cultured clinical samples. It therefore provides an additional tool to aid investigations into the potential spread of F89 strain throughout Europe and elsewhere.
Keyword Antimicrobial resistance
Spectrum cephalosporins
Pena gene
Cefixime
Susceptibility
Mutations
Markers
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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