Excitatory amino acid transporter 5 is widely expressed in peripheral tissues

Lee, A., Anderson, A. R., Stevens, M. G., Beasley, S., Barnett, N. L. and Pow, D. V. (2013) Excitatory amino acid transporter 5 is widely expressed in peripheral tissues. European Journal of Histochemistry, 57 1: 66-71. doi:10.4081/ejh.2013.e11

Author Lee, A.
Anderson, A. R.
Stevens, M. G.
Beasley, S.
Barnett, N. L.
Pow, D. V.
Title Excitatory amino acid transporter 5 is widely expressed in peripheral tissues
Journal name European Journal of Histochemistry   Check publisher's open access policy
ISSN 1121-760X
Publication date 2013-01
Year available 2013
Sub-type Article (original research)
DOI 10.4081/ejh.2013.e11
Open Access Status DOI
Volume 57
Issue 1
Start page 66
End page 71
Total pages 6
Place of publication Pavia, Italy
Publisher Pagepress
Collection year 2014
Language eng
Abstract It is routinely stated in the literature that Excitatory Amino Acid Transporter 5 (EAAT5) is a retina-specific glutamate transporter. EAAT5 is expressed by retinal photoreceptors and bipolar cells, where it serves as a slow transporter and as an inhibitory glutamate receptor, the latter role is due to the gating of a large chloride conductance. The dogma of an exclusively retinal distribution has arisen because Northern blot analyses have previously shown only modest hybridisation in non-retinal tissues. Others have re-interpreted this as indicating that EAAT5 was only present in retinal tissues. However, this view appears to be erroneous; recent evidence demonstrating abundant expression of EAAT5 in rat testis prompted us to re-examine this dogma. A new anti- body was developed to an intracellular loop region of rat EAAT5. This new tool, in concert with RT-PCR and sequencing, demonstrated that EAAT5 is widely distributed at the mRNA and protein levels in many non-nervous tissues including liver, kidney, intestine, heart, lung, and skeletal muscle. We conclude that EAAT5 is a widely distributed protein. Whether it functions in all locations as a glutamate transporter, or mainly as a glutamate-gated chloride conductance, remains to be determined.
Keyword EAAT5
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2014 Collection
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Citation counts: TR Web of Science Citation Count  Cited 2 times in Thomson Reuters Web of Science Article | Citations
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