Protocol for the use of enzyme-linked hybridization assays for genital ulcer disease

Bialasiewicz, Seweryn, Mackay, Ian M., Whiley, David M. and Sloots, Theo P. (2012). Protocol for the use of enzyme-linked hybridization assays for genital ulcer disease. In Colin R. MacKenzie and Birgit Henrich (Ed.), Diagnosis of sexually transmitted diseases (pp. 225-233) New York, NY, United States: Humana Press. doi:10.1007/978-1-61779-937-2_14

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Author Bialasiewicz, Seweryn
Mackay, Ian M.
Whiley, David M.
Sloots, Theo P.
Title of chapter Protocol for the use of enzyme-linked hybridization assays for genital ulcer disease
Title of book Diagnosis of sexually transmitted diseases
Place of Publication New York, NY, United States
Publisher Humana Press
Publication Year 2012
Sub-type Chapter in textbook
DOI 10.1007/978-1-61779-937-2_14
Year available 2012
Series Methods in Molecular Biology
ISBN 9781617799365
9781617799372
ISSN 1940-6029
1064-3745
Editor Colin R. MacKenzie
Birgit Henrich
Volume number 903
Chapter number 14
Start page 225
End page 233
Total pages 9
Total chapters 29
Collection year 2013
Language eng
Abstract/Summary Etiologic agents of genital ulcer disease include herpes simplex 1 and 2, Treponema pallidum pallidum, Haemophilus ducreyi, and Klebsiella granulomatis. The advent of PCR has allowed for more rapid and sensitive detection of microbial pathogens. In this protocol, we describe the simultaneous detection of these five pathogens and an internal control using a single-tube multiplex PCR and colorimetric enzyme-linked amplicon hybridization assay.
Q-Index Code BX
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Book Chapter
Collections: Non HERDC
School of Chemistry and Molecular Biosciences
 
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Created: Tue, 30 Apr 2013, 11:29:43 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences