Phenotypes and Karyotypes of Human Malignant Mesothelioma Cell Lines

Relan, Vandana, Morrison, Leanne, Parsonson, Kylie, Clarke, Belinda E., Duhig, Edwina E., Windsor, Morgan N., Matar, Kevin S., Naidoo, Rishendran, Passmore, Linda, McCaul, Elizabeth, Courtney, Deborah, Yang, Ian A., Fong, Kwun M. and Bowman, Rayleen V. (2013) Phenotypes and Karyotypes of Human Malignant Mesothelioma Cell Lines. Plos One, 8 3: e58132.1-e58132.8. doi:10.1371/journal.pone.0058132


Author Relan, Vandana
Morrison, Leanne
Parsonson, Kylie
Clarke, Belinda E.
Duhig, Edwina E.
Windsor, Morgan N.
Matar, Kevin S.
Naidoo, Rishendran
Passmore, Linda
McCaul, Elizabeth
Courtney, Deborah
Yang, Ian A.
Fong, Kwun M.
Bowman, Rayleen V.
Title Phenotypes and Karyotypes of Human Malignant Mesothelioma Cell Lines
Journal name Plos One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2013-03
Sub-type Article (original research)
DOI 10.1371/journal.pone.0058132
Open Access Status DOI
Volume 8
Issue 3
Start page e58132.1
End page e58132.8
Total pages 8
Place of publication San Francisco, CA United States
Publisher Public Library of Science
Collection year 2014
Language eng
Formatted abstract
Background: Malignant mesothelioma is an aggressive tumour of serosal surfaces most commonly pleura. Characterised cell lines represent a valuable tool to study the biology of mesothelioma. The aim of this study was to develop and biologically characterise six malignant mesothelioma cell lines to evaluate their potential as models of human malignant mesothelioma.

Methods: Five lines were initiated from pleural biopsies, and one from pleural effusion of patients with histologically proven malignant mesothelioma. Mesothelial origin was assessed by standard morphology, Transmission Electron Microscopy (TEM) and immunocytochemistry. Growth characteristics were assayed using population doubling times. Spectral karyotyping was performed to assess chromosomal abnormalities. Authentication of donor specific derivation was undertaken by DNA fingerprinting using a panel of SNPs.

Results: Most of cell lines exhibited spindle cell shape, with some retaining stellate shapes. At passage 2 to 6 all lines stained positively for calretinin and cytokeratin 19, and demonstrated capacity for anchorage-independent growth. At passage 4 to 16, doubling times ranged from 30-72 hours, and on spectral karyotyping all lines exhibited numerical chromosomal abnormalities ranging from 41 to 113. Monosomy of chromosomes 8, 14, 22 or 17 was observed in three lines. One line displayed four different karyotypes at passage 8, but only one karyotype at passage 42, and another displayed polyploidy at passage 40 which was not present at early passages. At passages 5-17, TEM showed characteristic features of mesothelioma ultrastructure in all lines including microvilli and tight intercellular junctions.

Conclusion: These six cell lines exhibit varying cell morphology, a range of doubling times, and show diverse passage-dependent structural chromosomal changes observed in malignant tumours. However they retain characteristic immunocytochemical protein expression profiles of mesothelioma during maintenance in artificial culture systems. These characteristics support their potential as in vitro model systems for studying cellular, molecular and genetic aspects of mesothelioma.
Keyword Comparative Genomic Hybridization
Copy number changes
Pleural Mesothelioma
Chromosomal Abnormalities
Tumorigenicity
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Medicine Publications
 
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