Quantitative PCR confirms culture as the gold standard for detection of lower airway infection by nontypeable Haemophilus influenzae in Australian Indigenous children with bronchiectasis

Hare, Kim M., Marsh, Robyn L., Binks, Michael J., Grimwood, Keith, Pizzutto, Susan J., Leach, Amanda J., Chang, Anne B. and Smith-Vaughan, Heidi C. (2013) Quantitative PCR confirms culture as the gold standard for detection of lower airway infection by nontypeable Haemophilus influenzae in Australian Indigenous children with bronchiectasis. Journal of Microbiological Methods, 92 3: 270-272. doi:10.1016/j.mimet.2012.12.013


Author Hare, Kim M.
Marsh, Robyn L.
Binks, Michael J.
Grimwood, Keith
Pizzutto, Susan J.
Leach, Amanda J.
Chang, Anne B.
Smith-Vaughan, Heidi C.
Title Quantitative PCR confirms culture as the gold standard for detection of lower airway infection by nontypeable Haemophilus influenzae in Australian Indigenous children with bronchiectasis
Journal name Journal of Microbiological Methods   Check publisher's open access policy
ISSN 0167-7012
1872-8359
Publication date 2013-03
Year available 2012
Sub-type Article (original research)
DOI 10.1016/j.mimet.2012.12.013
Volume 92
Issue 3
Start page 270
End page 272
Total pages 3
Place of publication Amsterdam, AE, Netherlands
Publisher Elsevier BV
Collection year 2013
Language eng
Formatted abstract
Correlation was observed between quantitative PCR and semi-quantitative culture for definition of Haemophilus influenzae infection in bronchoalveolar lavage specimens from 81 children with bronchiectasis. However, qPCR data correlated less well with airway neutrophilia, and supports continued use of culture as the gold standard for defining H. influenzae lower airway infection.

We investigated methods to define Haemophilus influenzae lower airway infection. ► We found good correlation between quantitative PCR and semi-quantitative culture. ► qPCR confirmed NTHi infection in specimens with concurrent Haemophilus haemolyticus. ► However qPCR correlated less well than culture with airway inflammatory indices. ► Our data support the continued use of quantitative culture as the gold standard.

Keyword Airway cellularity
Bronchiectasis
Estimates of infection
Haemophilus haemolyticus
Lower airway infection
Nontypeable Haemophilus influenzae
Streptococcus-Pneumoniae
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 22 December 2012

Document type: Journal Article
Sub-type: Article (original research)
Collections: Faculty of Health and Behavioural Sciences -- Publications
Official 2013 Collection
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 3 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 3 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 28 Apr 2013, 00:20:56 EST by System User on behalf of School of Medicine