Urine metabonomic profiling of a female adolescent with PIT-1 mutation before and during growth hormone therapy: insights into the metabolic effects of growth hormone

Abd Rahman, Shaffinaz, Schirra, Horst Joachim, Lichanska, Agnieszka M., Huynh, Tony and Leong, Gary M. (2013) Urine metabonomic profiling of a female adolescent with PIT-1 mutation before and during growth hormone therapy: insights into the metabolic effects of growth hormone. Growth Hormone & IGF Research, 23 1-2: 29-36. doi:10.1016/j.ghir.2012.12.001


Author Abd Rahman, Shaffinaz
Schirra, Horst Joachim
Lichanska, Agnieszka M.
Huynh, Tony
Leong, Gary M.
Title Urine metabonomic profiling of a female adolescent with PIT-1 mutation before and during growth hormone therapy: insights into the metabolic effects of growth hormone
Journal name Growth Hormone & IGF Research   Check publisher's open access policy
ISSN 1096-6374
1532-2238
Publication date 2013-02
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.ghir.2012.12.001
Open Access Status
Volume 23
Issue 1-2
Start page 29
End page 36
Total pages 8
Place of publication London, United Kingdom
Publisher Churchill Livingstone
Collection year 2014
Language eng
Formatted abstract
Objective: Growth hormone (GH) is a protein hormone with important roles in growth and metabolism. The objective of this study was to investigate the metabolism of a human subject with severe GH deficiency (GHD) due to a PIT-1 gene mutation and the metabolic effects of GH therapy using Nuclear Magnetic Resonance (NMR)-based metabonomics. NMR-based metabonomics is a platform that allows the metabolic profile of biological fluids such as urine to be recorded, and any alterations in the profile modulated by GH can potentially be detected.

Design: Urine samples were collected from a female subject with severe GHD before, during and after GH therapy, and from healthy age- and sex-matched controls and analysed with NMR-based metabonomics.

Setting: The samples were collected at a hospital and the study was performed at a research facility.

Participants: We studied a 17. year old female adolescent with severe GHD secondary to PIT-1 gene mutation who had reached final adult height and who had ceased GH therapy for over 3. years. The subject was subsequently followed for 5. years with and without GH therapy. Twelve healthy age-matched female subjects acted as control subjects.

Intervention: The GH-deficient subject re-commenced GH therapy at a dose of 1. mg/day to normalise serum IGF-1 levels. Main outcome measures: Urine metabolic profiles were recorded using NMR spectroscopy and analysed with multivariate statistics to distinguish the profiles at different time points and identify significant metabolites affected by GH therapy.

Results: NMR-based metabonomics revealed that the metabolic profile of the GH-deficient subject altered with GH therapy and that her profile was different from healthy controls before, and during withdrawal of GH therapy.

Conclusion: This study illustrates the potential use of NMR-based metabonomics for monitoring the effects of GH therapy on metabolism by profiling the urine of GH-deficient subjects. Further controlled studies in larger numbers of GH-deficient subjects are required to determine the clinical benefits of NMR-based metabonomics in subjects receiving GH therapy.
Keyword NMR
Metabonomics
Growth hormone
PIT-1
Urine
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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