Polymer–peptide hybrids as a highly immunogenic single-dose nanovaccine

Ahmad Fuaad, Abdullah A. H., Jia, Zhongfan, Hartas, Jon, Ziora, Zyta M., Lin, I-Chun, Moyle, Peter M., Batzloff, Michael R., Good, Michael F., Monteiro, Michael J., Skwarczynski, Mariusz and Toth, Istvan (2014) Polymer–peptide hybrids as a highly immunogenic single-dose nanovaccine. Nanomedicine, 9 1: 35-43. doi:10.2217/NNM.13.7


Author Ahmad Fuaad, Abdullah A. H.
Jia, Zhongfan
Hartas, Jon
Ziora, Zyta M.
Lin, I-Chun
Moyle, Peter M.
Batzloff, Michael R.
Good, Michael F.
Monteiro, Michael J.
Skwarczynski, Mariusz
Toth, Istvan
Title Polymer–peptide hybrids as a highly immunogenic single-dose nanovaccine
Journal name Nanomedicine   Check publisher's open access policy
ISSN 1743-5889
1748-6963
Publication date 2014
Year available 2013
Sub-type Article (original research)
DOI 10.2217/NNM.13.7
Volume 9
Issue 1
Start page 35
End page 43
Total pages 9
Place of publication London, United Kingdom
Publisher Future Medicine
Collection year 2014
Language eng
Formatted abstract
Aim: To explore four-arm star poly(t-butyl)acrylate (PtBA)–peptide and linear PtBA–peptide conjugates as a vaccine-delivery system against Group A Streptococcus.

Materials & methods:
PtBA nanoparticles bearing J14 peptide epitopes were prepared via alkyne-azide 1,3-dipolar cycloaddition ‘click’ reaction. The conjugated products were self-assembled into small or large nanoparticles. These nanoparticle vaccine candidates were evaluated in vivo and J14-specific antibody titers were assessed.

Results & discussion: Mice vaccinated with the nanoparticles were able to produce J14-specific IgG antibodies without the use of an external adjuvant after a single immunization. We have demonstrated for the first time that the immune responses against self-assembled PtBA nanoparticles are stronger for the smaller sized (~20 nm) nanoparticles compared with the larger (~500nm) PtBA nanoparticles.

Conclusion: PtBA analogs have the potential to be developed as potent carrier systems for single-dose synthetic vaccines.
Keyword Nanoparticle
Particle size
Peptide-based subunit vaccine
Poly(t-butyl)acrylate
Self-adjuvanting vaccine
Single immunization
Streptococcus pyogenes
Vaccine delivery
Vaccine formulation
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Posted online: 23 April 2013.

 
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Created: Fri, 26 Apr 2013, 10:08:51 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences