Possible mechanisms underlying the protective effects of SY-21, an extract of a traditional Chinese herb, on transient brain ischemia/reperfusion- induced neuronal death in rat hippocampus

Chen, Min, Wang, Yao, Liu, Yong, Hou, Xiao-Yu, Zhang, Quan-Guang, Meng, Fan-Jie and Zhang, Guang-Yi (2003) Possible mechanisms underlying the protective effects of SY-21, an extract of a traditional Chinese herb, on transient brain ischemia/reperfusion- induced neuronal death in rat hippocampus. Brain Research, 989 2: 180-186. doi:10.1016/S0006-8993(03)03331-6


Author Chen, Min
Wang, Yao
Liu, Yong
Hou, Xiao-Yu
Zhang, Quan-Guang
Meng, Fan-Jie
Zhang, Guang-Yi
Title Possible mechanisms underlying the protective effects of SY-21, an extract of a traditional Chinese herb, on transient brain ischemia/reperfusion- induced neuronal death in rat hippocampus
Journal name Brain Research   Check publisher's open access policy
ISSN 0006-8993
1872-6240
Publication date 2003-11-07
Sub-type Correction/erratum
DOI 10.1016/S0006-8993(03)03331-6
Volume 989
Issue 2
Start page 180
End page 186
Total pages 7
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Abstract We examined the neuroprotective actions of SY-21, a potent ingredient of a traditional Chinese herb, histologically. Transient brain ischemia (15 min) was induced by four-vessel occlusion in Sprague-Dawley rats. Administration of SY-21 20 min before or 6 h after brain ischemia significantly increased the number of surviving hippocampal CA1 pyramidal cells in rats subjected to transient brain ischemia followed by 5 days of reperfusion. Neuronal cell death resulting from ischemic events is associated with abnormal activation of the NMDA receptors. Tyrosine phosphorylation of the NMDA receptor subunit NR2A by Src or Fyn has been implicated in the up-regulation of NMDA receptor activity. In order to investigate the possible mechanism of the neuroprotective action of SY-21, we examined the effects of SY-21 on the ischemia/reperfusion-induced increases in tyrosine phosphorylation of the NMDA receptor subunit 2A (NR2A) and on the interactions involving NR2A, PSD-95 and Src/Fyn. We found that the increase in the tyrosine phosphorylation of NR2A induced by brain ischemia/reperfusion is suppressed by SY-21 administered 15 min before, or instantly after, brain ischemia. Also, SY-21 attenuated the increased interactions involving NR2A, PSD-95, Fyn and Src. These results demonstrate that SY-21 has a prominent neuroprotective action against brain ischemic insult, and the mechanism may involve the regulation of the tyrosine phosphorylation of NR2A by changing the above interactions.
Keyword Brain ischemia
Hippocampus
Neuroprotective action
NMDA receptor
Rat
SY-21
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Correction/erratum
Collection: Centre for Advanced Imaging Publications
 
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Created: Tue, 23 Apr 2013, 19:49:05 EST by Sandrine Ducrot on behalf of Centre for Advanced Imaging