Influence of prior mode of growth on physiological properties of Campylobacter cells

Nguyen, Vu Tuan, Fegan, Narelle, Turner, Mark S. and Dykes, Gary A. (2012). Influence of prior mode of growth on physiological properties of Campylobacter cells. In: Glenn Graham, Michael Netzel, Trudy Graham, Mary Fletcher, Gloria Karagianis, Cindy Giles, Ujang Tinggi and Pieter Scheelings, Technology for Food Quality: Proceedings 12th Government Food Analysts Conference. 12th Government Food Analysts Conference, Brisbane, Australia, (44-52). 22-24 February 2011.

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Name Description MIMEType Size Downloads
Author Nguyen, Vu Tuan
Fegan, Narelle
Turner, Mark S.
Dykes, Gary A.
Title of paper Influence of prior mode of growth on physiological properties of Campylobacter cells
Formatted title
Influence of prior mode of growth on physiological properties of Campylobacter cells
Conference name 12th Government Food Analysts Conference
Conference location Brisbane, Australia
Conference dates 22-24 February 2011
Proceedings title Technology for Food Quality: Proceedings 12th Government Food Analysts Conference
Place of Publication Coopers Plains, QLD, Australia
Publisher Health and Food Sciences Precinct (HFSP)
Publication Year 2012
Sub-type Fully published paper
ISBN 9780977596867
Editor Glenn Graham
Michael Netzel
Trudy Graham
Mary Fletcher
Gloria Karagianis
Cindy Giles
Ujang Tinggi
Pieter Scheelings
Start page 44
End page 52
Total pages 9
Collection year 2013
Language eng
Formatted Abstract/Summary
Campylobacter is an important foodborne pathogen of worldwide concern. Planktonic (broth) or sessile (agar) modes of growth have been suggested to affect cell physiology and virulence of pathogenic bacteria. This work aimed to investigate the influence of prior mode of growth (planktonic and sessile culture) on selected physiological properties of cells of two Campylobacter strains (one Campylobacter jejuni strain and one C. coli strain). Specifically, cell surface hydrophobicity, surface charge, capsular polysaccharide (CPS) and lipooligosaccharide (LOS) expression and quantification, autoagglutination and adhesiveness were examined. Cell surface hydrophobicity, surface charge and autoagglutination were determined by water contact angle, zeta potential and optical density measurements, respectively. Expression of CPS and LOS were determined using SDS-PAGE with Alcian blue staining and quantified using an acid hydrolysis method. The adhesiveness of Campylobacter was evaluated based on the numbers of cells attaching to stainless steel and glass. There was no significant (P>0.05) difference between planktonic and sessile cells with regards to zeta potential values, indicating prior mode of growth may not affect cell surface charge of Campylobacter. Cells grown under both culture conditions demonstrated similar CPS and LOS profiles and produced comparable (P>0.05) amounts of CPS and LOS, suggesting growing Campylobacter in either planktonic or sessile mode may not influence CPS and LOS expression and quantification. However, cells exhibited a significantly (P<0.05) higher degree of hydrophobicity, autoagglutination activity and attachment to stainless steel and glass when grown in planktonic culture than in sessile culture. These findings suggest prior mode of growth strongly affects the physiology of Campylobacter.
Keyword Campylobacter
Planktonic
Sessile
Hydrophobicity
Adhesivenes
Autoagglutination
Q-Index Code E1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Conference Paper
Collections: School of Agriculture and Food Sciences
Official 2013 Collection
 
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Created: Mon, 22 Apr 2013, 11:36:25 EST by Ms Ramona Hooyer on behalf of School of Agriculture and Food Sciences