Engineering Deinococcus radiodurans into biosensor to monitor radioactivity and genotoxicity in environment

GuanJun, Gao, Lu, Fan, HuiMing, Lu and YueJin, Hua (2008) Engineering Deinococcus radiodurans into biosensor to monitor radioactivity and genotoxicity in environment. Chinese Science Bulletin, 53 11: 1675-1681. doi:10.1007/s11434-008-0224-6

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Author GuanJun, Gao
Lu, Fan
HuiMing, Lu
YueJin, Hua
Title Engineering Deinococcus radiodurans into biosensor to monitor radioactivity and genotoxicity in environment
Formatted title
Engineering Deinococcus radiodurans into biosensor to monitor radioactivity and genotoxicity in environment
Journal name Chinese Science Bulletin   Check publisher's open access policy
ISSN 1001-6538
2095-9281
Publication date 2008-06-01
Sub-type Article (original research)
DOI 10.1007/s11434-008-0224-6
Open Access Status File (Publisher version)
Volume 53
Issue 11
Start page 1675
End page 1681
Total pages 7
Place of publication Beijing, China
Publisher Science in China Press
Language eng
Formatted abstract
Based on a genetically modified radioresistant bacteria Deinococcus radiodurans, we constructed a real time whole cell biosensor to monitor radioactivity and genotoxicity in highly radioactive environment. The enhanced green fluorescence protein (eGFP) was fused to the promoter of the crucial DNA damage-inducible recA gene from D. radiodurans, and the consequent DNA fragment (PrecA-egfp) carried by plasmid was introduced into D. radiodurans R1 strain to obtain the biosensor strain DRG300. This engineered strain can express eGFP protein and generate fluorescence in induction of the recA gene promoter. Based on the correlation between fluorescence intensity and protein expression level in live D. radiodurans cells, we discovered that the fluorescence induction of strain DRG300 responds in a remarkable dose-dependent manner when treated with DNA damage sources such as gamma radiation and mitomycin C. It is encouraging to find the widely detective range and high sensitivity of this reconstructed strain comparing with other whole cell biosensors in former reports. These results suggest that the strain DRG300 is a potential whole cell biosensor to construct a detective system to monitor the biological hazards of radioactive and toxic pollutants in environment in real time.
Keyword Deinococcus radiodurans
Biosensor
eGFP
RecA
Genotoxicity
DNA damage
Ionizing radiation
Mitomycin C
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Advanced Water Management Centre Publications
 
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Created: Thu, 18 Apr 2013, 00:42:42 EST by Chris Fan on behalf of Advanced Water Management Centre