MRI changes and complement activation correlate with epileptogenicity in a mouse model of temporal lobe epilepsy

Kharatishvili, Irina, Shan, Zuyao Y., She, David T., Foong, Samuel, Kurniawan, Nyoman D. and Reutens, David C. (2014) MRI changes and complement activation correlate with epileptogenicity in a mouse model of temporal lobe epilepsy. Brain Structure and Function, 219 2: 683-706. doi:10.1007/s00429-013-0528-4

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Author Kharatishvili, Irina
Shan, Zuyao Y.
She, David T.
Foong, Samuel
Kurniawan, Nyoman D.
Reutens, David C.
Title MRI changes and complement activation correlate with epileptogenicity in a mouse model of temporal lobe epilepsy
Journal name Brain Structure and Function   Check publisher's open access policy
ISSN 1863-2653
Publication date 2014-03
Year available 2013
Sub-type Article (original research)
DOI 10.1007/s00429-013-0528-4
Open Access Status File (Author Post-print)
Volume 219
Issue 2
Start page 683
End page 706
Total pages 24
Place of publication Heidelberg, Germany
Publisher Springer
Collection year 2014
Language eng
Abstract The complex pathogenesis of temporal lobe epilepsy includes neuronal and glial pathology, synaptic reorganization, and an immune response. However, the spatio-temporal pattern of structural changes in the brain that provide a substrate for seizure generation and modulate the seizure phenotype is yet to be completely elucidated. We used quantitative magnetic resonance imaging (MRI) to study structural changes triggered by status epilepticus (SE) and their association with epileptogenesis and with activation of complement component 3 (C3). SE was induced by injection of pilocarpine in CD1 mice. Quantitative diffusion-weighted imaging and T2 relaxometry was performed using a 16.4-Tesla MRI scanner at 3 h and 1, 2, 7, 14, 28, 35, and 49 days post-SE. Following longitudinal MRI examinations, spontaneous recurrent seizures and interictal spikes were quantified using continuous video-EEG monitoring. Immunohistochemical analysis of C3 expression was performed at 48 h, 7 days, and 4 months post-SE. MRI changes were dynamic, reflecting different outcomes in relation to the development of epilepsy. Apparent diffusion coefficient changes in the hippocampus at 7 days post-SE correlated with the severity of the evolving epilepsy. C3 activation was found in all stages of epileptogenesis within the areas with significant MRI changes and correlated with the severity of epileptic condition.
Keyword Complement
Epilepsy animal models
Magnetic resonance imaging
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online 10 March 2013

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
Centre for Advanced Imaging Publications
UQ Diamantina Institute Publications
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Citation counts: TR Web of Science Citation Count  Cited 7 times in Thomson Reuters Web of Science Article | Citations
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Created: Wed, 17 Apr 2013, 13:36:04 EST by Sandrine Ducrot on behalf of Centre for Advanced Imaging