Cloning and expression of porcine Colony Stimulating Factor-1 (CSF-1) and Colony Stimulating Factor-1 Receptor (CSF-1R) and analysis of the species specificity of stimulation by CSF-1 and Interleukin 34

Gow, Deborah J., Garceau, Valerie, Kapetanovic, Ronan, Sester, David P., Fici, Greg J., Shelly, John A., Wilson, Thomas L. and Hume, David A. (2012) Cloning and expression of porcine Colony Stimulating Factor-1 (CSF-1) and Colony Stimulating Factor-1 Receptor (CSF-1R) and analysis of the species specificity of stimulation by CSF-1 and Interleukin 34. Cytokine, 60 3: 793-805. doi:10.1016/j.cyto.2012.08.008


Author Gow, Deborah J.
Garceau, Valerie
Kapetanovic, Ronan
Sester, David P.
Fici, Greg J.
Shelly, John A.
Wilson, Thomas L.
Hume, David A.
Title Cloning and expression of porcine Colony Stimulating Factor-1 (CSF-1) and Colony Stimulating Factor-1 Receptor (CSF-1R) and analysis of the species specificity of stimulation by CSF-1 and Interleukin 34
Journal name Cytokine   Check publisher's open access policy
ISSN 1043-4666
1096-0023
Publication date 2012-12
Year available 2012
Sub-type Article (original research)
DOI 10.1016/j.cyto.2012.08.008
Volume 60
Issue 3
Start page 793
End page 805
Total pages 13
Place of publication London, United Kingdom
Publisher Academic Press
Collection year 2013
Language eng
Formatted abstract
Macrophage Colony Stimulating Factor (CSF-1) controls the survival, differentiation and proliferation of cells of the mononuclear phagocyte system. A second ligand for the CSF-1R, Interleukin 34 (IL-34), has been described, but its physiological role is not yet known. The domestic pig provides an alternative to traditional rodent models for evaluating potential therapeutic applications of CSF-1R agonists and antagonists. To enable such studies, we cloned and expressed active pig CSF-1. To provide a bioassay, pig CSF-1R was expressed in the factor-dependent Ba/F3 cell line. On this transfected cell line, recombinant porcine CSF-1 and human CSF-1 had identical activity. Mouse CSF-1 does not interact with the human CSF-1 receptor but was active on pig. By contrast, porcine CSF-1 was active on mouse, human, cat and dog cells. IL-34 was previously shown to be species-specific, with mouse and human proteins demonstrating limited cross-species activity. The pig CSF-1R was equally responsive to both mouse and human IL-34. Based upon the published crystal structures of SF-1/CSF-1R and IL34/CSF-1R complexes, we discuss the molecular basis for the species specificity.
Keyword Macrophage
Ba/F3 cells
Bone marrow
Hematopoiesis
Species specificity
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
Institute for Molecular Bioscience - Publications
 
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Created: Tue, 16 Apr 2013, 15:02:59 EST by Susan Allen on behalf of Institute for Molecular Bioscience