Dynamic changes in localization of chromobox (CBX) family members during the maternal to embryonic transition

Ruddock-D'Cruz, Nancy T., Prashadkumar, Sivachelvi, Wilson, Katrina J., Heffernan, Corey, Cooney, Melissa A., French, Andrew J., Jans, David A., Verma, Paul J. and Holland, Michael K. (2008) Dynamic changes in localization of chromobox (CBX) family members during the maternal to embryonic transition. Molecular Reproduction and Development, 75 3: 477-488. doi:10.1002/mrd.20752


Author Ruddock-D'Cruz, Nancy T.
Prashadkumar, Sivachelvi
Wilson, Katrina J.
Heffernan, Corey
Cooney, Melissa A.
French, Andrew J.
Jans, David A.
Verma, Paul J.
Holland, Michael K.
Title Dynamic changes in localization of chromobox (CBX) family members during the maternal to embryonic transition
Journal name Molecular Reproduction and Development   Check publisher's open access policy
ISSN 1040-452X
1098-2795
Publication date 2008-03
Year available 2007
Sub-type Article (original research)
DOI 10.1002/mrd.20752
Volume 75
Issue 3
Start page 477
End page 488
Total pages 12
Place of publication Hoboken, NJ, United States
Publisher John Wiley & Sons
Language eng
Abstract The Chromobox domain (Cbx) gene family, consisting of Polycomb and Heterochromatin Protein 1 genes, is involved in transcriptional repression, cell cycle regulation and chromatin remodeling. We report the first study of gene expression and protein localization of the Cbx genes in in vitro produced bovine embryos. All but one gene (Cbx6) were expressed. This was confirmed by immunolocalization for HP1α, β, γ, and Pc2, 3. HP1β was found in the nuclei of embryos from the two-cell stage onwards, whereas HP1γ showed diffuse cytoplasmic/nuclear localization at the two- and eight-cell stages, and predominantly nuclear localization at the four-cell stage and the 16-cell stage onwards. Leptomycin B (LMB), a specific inhibitor of the nuclear export protein CRM-1 (chromosomal regional maintenance-1), was found to increase nuclear localization of HP1γ at the eight-cell stage, and to prevent progression past this stage of embryogenesis. This indicates that HP1γ possesses a CRM-1-dependent nuclear export pathway which may represent part of the basis of HP1γ's ability to shuttle between the nucleus and the cytoplasm in dynamic fashion. HP1α was expressed in embryonic nuclei at all stages, but was found to relocalise from euchromatin to heterochromatin during the maternal to embryonic transition (MET). In contrast, Pc2 and Pc3 were evenly distributed between cytoplasm and nucleus until the eight- and sixteen-cell stages or the morula stage, respectively, before relocating preferentially to the cytoplasm. Collectively, the results suggest that dynamic changes of the nuclear-cytoplasmic and subnuclear distribution of members of the Cbx family may be central to the MET.
Keyword Embryo
Bovine
MET
Embryonic genome activation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Veterinary Science Publications
 
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Created: Mon, 15 Apr 2013, 14:59:07 EST by Dr Michael Holland on behalf of School of Veterinary Science