Mitochondrial DNA transmission and transcription after somatic cell fusion to one or more cytoplasts

Bowles, Emma J., Tercirlioglu, R. Tayfur, French, Andrew J., Holland, Michael K. and St John, Justin C. (2008) Mitochondrial DNA transmission and transcription after somatic cell fusion to one or more cytoplasts. Stem Cells, 26 3: 775-782. doi:10.1634/stemcells.2007-0747


Author Bowles, Emma J.
Tercirlioglu, R. Tayfur
French, Andrew J.
Holland, Michael K.
St John, Justin C.
Title Mitochondrial DNA transmission and transcription after somatic cell fusion to one or more cytoplasts
Journal name Stem Cells   Check publisher's open access policy
ISSN 1066-5099
1549-4918
Publication date 2008-03
Year available 2008
Sub-type Article (original research)
DOI 10.1634/stemcells.2007-0747
Volume 26
Issue 3
Start page 775
End page 782
Total pages 8
Place of publication Durham, NC, United States
Publisher AlphaMed Press
Language eng
Abstract Following fertilization, mitochondrial DNA is inherited from the oocyte and transmitted homoplasmically. However, following nuclear transfer, mitochondrial DNA can be transmitted from both the donor cell and recipient oocyte, resulting in a state of heteroplasmy. To determine whether the genetic diversity between donor cell and recipient cytoplast mitochondrial DNA influences development, we generated bovine embryos by fusing a donor cell to one or more enucleated cytoplasts. Analysis of mitochondrial DNA from embryos, fetal tissues, and blood samples from offspring revealed that early preimplantation embryos from two or three cytoplasts had significantly more mitochondrial DNA variants than fetal tissues. Phylogenic analysis of embryos generated using single cytoplasts divided the mitochondrial DNA sequence variants into three separate groups with various amounts of genetic divergence from the donor cell line. In heteroplasmic tissue and blood samples, the predominant mitochondrial DNA population was significantly more divergent from the donor cell than the less frequent allele. Furthermore, analysis of the mitochondrially encoded cytochrome B gene showed that two heteroplasmic alleles encoded for different amino acids, and the ratios of mitochondrial DNA/mRNA for each allele differed significantly between tissues. The degree of evolutionary distance between the donor cell and the cytoplast and the variability in heteroplasmy between tissues may have an impact on more divergent intergeneric nuclear transfer and the use of this approach for the generation of embryonic stem cells.
Keyword Cloning
Evolutionary distance
Heteroplasmy
Mitochondrial DNA
Replication
Transcription
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Veterinary Science Publications
 
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Created: Mon, 15 Apr 2013, 14:49:58 EST by Dr Michael Holland on behalf of School of Veterinary Science