The generation of live offspring from vitrified oocytes

Sanchez-Partilda, L. Gabriel, Kelly, Richard D. W., Sumner, Huseyin, Lo, Camden Y., Aharon, Rotem, Holland, Michael K., O'Bryan, Moira K. and St John, Justin C. (2011) The generation of live offspring from vitrified oocytes. PLoS One, 6 6: e21597.1-e21597.11. doi:10.1371/journal.pone.0021597

Author Sanchez-Partilda, L. Gabriel
Kelly, Richard D. W.
Sumner, Huseyin
Lo, Camden Y.
Aharon, Rotem
Holland, Michael K.
O'Bryan, Moira K.
St John, Justin C.
Title The generation of live offspring from vitrified oocytes
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2011-06
Year available 2011
Sub-type Article (original research)
DOI 10.1371/journal.pone.0021597
Open Access Status DOI
Volume 6
Issue 6
Start page e21597.1
End page e21597.11
Total pages 11
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Language eng
Abstract Oocyte cryopreservation is extremely beneficial for assisted reproductive technologies, the treatment of infertility and biotechnology and offers a viable alternative to embryo freezing and ovarian grafting approaches for the generation of embryonic stem cells and live offspring. It also offers the potential to store oocytes to rescue endangered species by somatic cell nuclear transfer and for the generation of embryonic stem cells to study development in these species. We vitrified mouse oocytes using a range of concentrations of trehalose (0 to 0.3 M) and demonstrated that 0.1 and 0.3 M trehalose had similar developmental rates, which were significantly different to the 0.2 M cohort (P<0.05). As mitochondria are important for fertilisation outcome, we observed that the clustering and distribution of mitochondria of the 0.2 M cohort were more affected by vitifrication than the other groups. Nevertheless, all 3 cohorts were able to develop to blastocyst, following in vitro fertilisation, although developmental rates were better for the 0.1 and 0.3 M cohorts than the 0.2 M cohort (P<0.05). Whilst blastocysts gave rise to embryonic stem-like cells, it was apparent from immunocytochemistry and RT-PCR that these cells did not demonstrate true pluripotency and exhibited abnormal karyotypes. However, they gave rise to teratomas following injection into SCID mice and differentiated into cells of each of the germinal layers following in vitro differentiation. The transfer of 2-cell embryos from the 0.1 and 0.3 M cohorts resulted in the birth of live offspring that had normal karyotypes (9/10). When 2-cell embryos from vitrified oocytes underwent vitrification, and were thawed and transferred, live offspring were obtained that exhibited normal karyotypes, with the exception of one offspring who was larger and died at 7 months. We conclude that these studies highlight the importance of the endometrial environment for the maintenance of genetic stability and thus the propagation of specific genetic traits.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Article # e21597

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Veterinary Science Publications
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Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 11 times in Scopus Article | Citations
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Created: Mon, 15 Apr 2013, 14:38:41 EST by Dr Michael Holland on behalf of School of Veterinary Science