In situ high throughput method for H2S detection during micro-scale wine fermentation

Winter, Gal and Curtin, Chris (2012) In situ high throughput method for H2S detection during micro-scale wine fermentation. Journal of Microbiological Methods, 91 1: 165-170. doi:10.1016/j.mimet.2012.08.003


Author Winter, Gal
Curtin, Chris
Title In situ high throughput method for H2S detection during micro-scale wine fermentation
Formatted title
In situ high throughput method for H2S detection during micro-scale wine fermentation
Journal name Journal of Microbiological Methods   Check publisher's open access policy
ISSN 0167-7012
1872-8359
Publication date 2012-10
Sub-type Article (original research)
DOI 10.1016/j.mimet.2012.08.003
Volume 91
Issue 1
Start page 165
End page 170
Total pages 6
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2013
Language eng
Formatted abstract
An in situ high throughput method for the detection of H2S during fermentation was developed. The method utilizes a redox reaction in which sulfide ion reduces methylene blue, leading to its decolourisation. Incorporation of methylene blue into the fermentation media allows real-time detection of H2S during fermentation and the generation of an H2S production profile. Kinetic parameters extracted from the H2S production profile can be used to characterise genetic factors affecting H2S production and differentiate between environmental conditions affecting it. The method, validated here for Saccharomyces cerevisiae, is suited for high throughput screening purposes by virtue of its simplicity and the ability to detect H2S in micro-scale fermentations.
Keyword H2S
Hydrogen sulfide
Fermentation
High throughput
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
Advanced Water Management Centre Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 6 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 6 times in Scopus Article | Citations
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Created: Mon, 15 Apr 2013, 09:44:39 EST by Gal Winter on behalf of Advanced Water Management Centre