Caffeine induces macroautophagy and confers a cytocidal effect on food spoilage yeast in combination with benzoic acid

Winter, Gal, Hazan, Reut, Bakalinsky, Alan T. and Abeliovich, Hagai (2008) Caffeine induces macroautophagy and confers a cytocidal effect on food spoilage yeast in combination with benzoic acid. Autophagy, 4 1: 28-36.

Author Winter, Gal
Hazan, Reut
Bakalinsky, Alan T.
Abeliovich, Hagai
Title Caffeine induces macroautophagy and confers a cytocidal effect on food spoilage yeast in combination with benzoic acid
Journal name Autophagy   Check publisher's open access policy
ISSN 1554-8627
1554-8635
Publication date 2008-01
Sub-type Article (original research)
Volume 4
Issue 1
Start page 28
End page 36
Total pages 9
Place of publication Austin, TX, United States
Publisher Landes Bioscience
Language eng
Formatted abstract
Weak organic acids are an important class of food preservatives that are particularly efficacious towards yeast and fungal spoilage. While acids with small aliphatic chains appear to function by acidification of the cytosol and are required at high concentrations to inhibit growth, more hydrophobic organic acids such as sorbic and benzoic acid have been suggested to function by perturbing membrane dynamics and are growth-inhibitory at much lower concentrations. We previously demonstrated that benzoic acid has selective effects on membrane trafficking in Saccharomyces cerevisiae. Benzoic acid selectively blocks macroautophagy in S. cerevisiae while acetic acid does not, and sorbic acid does so to a lesser extent. Indeed, while benzoic acid alone is cytostatic and starvation treatment alone is cytostatic, the combination of these treatments is cytocidal, because macroautophagy is essential for survival during nitrogen starvation. In this report, we demonstrate that Zygosaccharomyces bailii, a food spoilage yeast with relatively high resistance to weak acid stress, also exhibits a cytocidal response to the combination of benzoic acid and nitrogen starvation. In addition, we show that nitrogen starvation can be replaced by caffeine supplementation. Caffeine induces a starvation response that includes the induction of macroautophagy, and the combination of caffeine and benzoic acid is cytocidal, as predicted from the nitrogen starvation data.
Keyword Autophagy
Vacuole
Caffeine
Benzoic acid
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Advanced Water Management Centre Publications
 
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Created: Mon, 15 Apr 2013, 09:38:01 EST by Gal Winter on behalf of Advanced Water Management Centre