The human stratum corneum prevents small gold nanoparticle penetration and their potential toxic metabolic consequences

Liu, David C., Raphael, Anthony P., Sundh, Daniel, Grice, Jeffrey E., Soyer, H. Peter, Roberts, Michael S. and Prow, Tarl W. (2012) The human stratum corneum prevents small gold nanoparticle penetration and their potential toxic metabolic consequences. Journal of Nanomaterials, 2012 72176.1-72176.8. doi:10.1155/2012/721706

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Author Liu, David C.
Raphael, Anthony P.
Sundh, Daniel
Grice, Jeffrey E.
Soyer, H. Peter
Roberts, Michael S.
Prow, Tarl W.
Title The human stratum corneum prevents small gold nanoparticle penetration and their potential toxic metabolic consequences
Journal name Journal of Nanomaterials   Check publisher's open access policy
ISSN 1687-4110
1687-4129
Publication date 2012-01
Sub-type Article (original research)
DOI 10.1155/2012/721706
Open Access Status DOI
Volume 2012
Start page 72176.1
End page 72176.8
Total pages 8
Place of publication New York, NY United States
Publisher Hindawi Publishing Corporation
Collection year 2013
Language eng
Formatted abstract
Nanoparticles are being used in multiple applications, ranging from biomedical and skin care products (e.g., sunscreen) through to industrial manufacturing processes (e.g., water purification). The increase in exposure has led to multiple reports on nanoparticle penetration and toxicity. However, the correlation between nanoparticle size and its penetration without physical/chemical enhancers through the skin is poorly understood-with studies instead focusing primarily on skin penetration under disrupted conditions. In this paper, we investigate the penetration and metabolic effects of 10nm, 30nm, and 60nm gold nanoparticles within viable excised human skin after 24-hour exposure using multiphoton tomograph-fluorescence lifetime imaging microscopy. After 24 hour treatment with the 10, 30, and 60nm gold nanoparticles, there was no significant penetration detected below the stratum corneum. Furthermore, there were no changes in metabolic output (total NAD(P)H) in the viable epidermis posttreatment correlating with lack of penetration of nanoparticles. These results are significant for estimating topical nanoparticle exposure in humans where other model systems may overestimate the exposure of nanoparticles to the viable epidermis. Our data shows that viable human skin resists permeation of small nanoparticles in a size range that has been reported to penetrate deeply in other skin models.
Keyword Human skin
In vitro
Gold nanoparticle
Human stratum corneum
Industrial manufacturing process
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Medicine Publications
 
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Created: Sun, 14 Apr 2013, 11:41:51 EST by Nicole Lin on behalf of Medicine - Princess Alexandra Hospital