Lab-on-a-chip platforms based on highly sensitive nanophotonic Si biosensors for single nucleotide DNA testing

Sanchez del Rio, J., Carrascosa, L. G., Blanco, F. J., Moreno, M., Berganzo, J., Calle, A., Dominguez, C. and Lechuga, L. M. (2007). Lab-on-a-chip platforms based on highly sensitive nanophotonic Si biosensors for single nucleotide DNA testing. In: Joel A. Kubby and Graham T. Reed, Silicon Photonics II. Conference on Silicon Photonics II, San Jose, United States, (64771B.1-64771B.9). 22-25 January 2007. doi:10.1117/12.713977

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Author Sanchez del Rio, J.
Carrascosa, L. G.
Blanco, F. J.
Moreno, M.
Berganzo, J.
Calle, A.
Dominguez, C.
Lechuga, L. M.
Title of paper Lab-on-a-chip platforms based on highly sensitive nanophotonic Si biosensors for single nucleotide DNA testing
Conference name Conference on Silicon Photonics II
Conference location San Jose, United States
Conference dates 22-25 January 2007
Proceedings title Silicon Photonics II   Check publisher's open access policy
Journal name Proceedings of SPIE - International Society for Optical Engineering   Check publisher's open access policy
Place of Publication Bellingham, WA, United States
Publisher S P I E - International Society for Optical Engineering
Publication Year 2007
Sub-type Fully published paper
DOI 10.1117/12.713977
Open Access Status File (Publisher version)
ISBN 9780819465900
ISSN 0277-786X
Editor Joel A. Kubby
Graham T. Reed
Volume 6477
Start page 64771B.1
End page 64771B.9
Total pages 9
Language eng
Abstract/Summary In order to solve the drawbacks of sensitivity and portability in optical biosensors we have developed ultrasensitive and miniaturized photonic silicon sensors able to be integrated in a "lab-on-a-chip" microsystem platform. The sensors are integrated Mach-Zehnder interferometers based on TIR optical waveguides (Si/SiO2/Si3N4) of micro/nanodimensions. We have applied this biosensor for DNA testing and for detection of single nucleotide polymorphisms at BRCA-1 gene, involved in breast cancer development, without target labeling. The oligonucleotide probe is immobilized by covalent attachment to the sensor surface through silanization procedures. The hybridization was performed for different DNA target concentrations showing a lowest detection limit at 10 pM. Additionally, we have detected the hybridization of different concentrations of DNA target with two mismatching bases corresponding to a mutation of the BRCA-1 gene. Following the way of the lab-on-a-chip microsystem, integration with the microfluidics has been achieved by using a novel fabrication method of 3-D embedded microchannels using the polymer SU-8 as structural material. The optofluidic chip shows good performances for biosensing.
Keyword Biosensor
Mach-Zehnder interferometer
Silicon microtechnology
DNA biosensing
Microfluidics
Lab-on-a-chip microsystem
Q-Index Code E1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Article number 64771B

 
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