Nanomechanics for specific biological detection

Alvarez, M., Carrascosa, L. G., Tamayo, J., Calle, A. and Lechuga, L. M. (2003). Nanomechanics for specific biological detection. In: Robert Vajtai, Xavier Aymerich, Laszlo B. Kish and Angel Rubio, Nanotechnology. Conference on Nanotechnology, Maspalomas, Gran Canaria, Spain, (197-206). 19-21May 2003. doi:10.1117/12.498629

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Author Alvarez, M.
Carrascosa, L. G.
Tamayo, J.
Calle, A.
Lechuga, L. M.
Title of paper Nanomechanics for specific biological detection
Conference name Conference on Nanotechnology
Conference location Maspalomas, Gran Canaria, Spain
Conference dates 19-21May 2003
Proceedings title Nanotechnology   Check publisher's open access policy
Journal name Proceedings of SPIE - International Society for Optical Engineering   Check publisher's open access policy
Place of Publication Bellingham, WA United States
Publisher S P I E - International Society for Optical Engineering
Publication Year 2003
Year available 2003
Sub-type Fully published paper
DOI 10.1117/12.498629
Open Access Status File (Publisher version)
ISBN 0819449784
9780819449788
ISSN 0277-786X
1996-756X
Editor Robert Vajtai
Xavier Aymerich
Laszlo B. Kish
Angel Rubio
Volume 5118
Start page 197
End page 206
Total pages 10
Collection year 2004
Language eng
Formatted Abstract/Summary
Nanomechanical biosensors have emerged as a promising technology for measurement of biomolecular interactions. Among the advantages are direct detection without need of labelling with fluorescent or radioactive molecules, small sensor area, high sensitivity and suitability for integration using silicon technology. Here we present two important applications: i) study of DNA immobilization for nucleic acid detection and ii) direct detection of the harmful pesticide dichlorodiphenyltrichloroethane (DDT). Single-stranded oligonucleotides were derivatized with thiol molecules for self-assembly on the gold-coated side of a microcantilever. The geometry of the binding and the surface density were studied and controlled by mixing derivatized oligonucleotides with spacer self-assembled monolayers. The hybridization signals were smaller than 10% of the immobilization signal. The molecular mechanisms responsible of the nanomechanical response due to hybridization are discussed. On the other hand, herbicide DDT was detected by performing competitive assays, in which the cantilever was coated with a synthetic DDT hapten, and it was exposed to different ratios between the monoclonal antibody and the DDT. The relevance of this technique in functional genomics and environmental control will be discussed.
Keyword Nanotechnology
Biosensors
Microcantilevers
Nanomechanics
Q-Index Code E1
Q-Index Status Provisional Code
Institutional Status Non-UQ

 
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Created: Fri, 12 Apr 2013, 02:18:09 EST by Laura Garcia Carrascosa on behalf of Aust Institute for Bioengineering & Nanotechnology