The expression of the let-7 miRNA and Lin28 signalling pathway in human term gestational tissues

Chan, H.-W., Lappas, M., Yi Yee, S. W., Vaswani, K., Mitchell, M. D. and Rice, G. E. (2013) The expression of the let-7 miRNA and Lin28 signalling pathway in human term gestational tissues. Placenta, 34 5: 443-448. doi:10.1016/j.placenta.2013.02.008

Author Chan, H.-W.
Lappas, M.
Yi Yee, S. W.
Vaswani, K.
Mitchell, M. D.
Rice, G. E.
Title The expression of the let-7 miRNA and Lin28 signalling pathway in human term gestational tissues
Journal name Placenta   Check publisher's open access policy
ISSN 0143-4004
Publication date 2013
Sub-type Article (original research)
DOI 10.1016/j.placenta.2013.02.008
Volume 34
Issue 5
Start page 443
End page 448
Total pages 6
Place of publication London, United Kingdom
Publisher Elsevier
Collection year 2014
Language eng
Formatted abstract
Introduction: Labour and delivery are processes associated with inflammation within intrauterine and cervical tissues. The mechanisms that induce labour-associated changes and, in particular, the role of microRNAs (miRNAs) remain to be elucidated. MiRNAs are small non-coding RNAs that repress gene expression via mRNA degradation and translational repression. Let-7 miRNAs are negatively regulated by RNA-binding protein, Lin28, and both function downstream of NF-κB signalling. In non-gestational tissues, let-7 and Lin28 reportedy function as negative and positive regulators of IL-6 expression. We hypothesised that labour-associated inflammation involves the downregulation of let-7 miRNAs and upregulation of Lin28 expression.

Aim: To determine the expression of Lin28 protein and let-7 miRNA in human gestational tissue obtained before and after labour. Method: Gestational tissues were collected from women at term by Caesarean section with and without labour and following normal vaginal delivery (n = 6 per group). Protein and RNA was extracted and Lin28 and let-7 miRNA expression was measured by Western blotting and real-time PCR.

Results: The data obtained established that let-7 miRNA and Lin28 display tissue-specific expression: Lin28 was strongly expressed in the placenta and choriodecidua, but not measurable in amnion; and let-7b and -7c expression were significantly lower in choriodecidua compare to placenta and amnion, whereas the amnion expressed less let-7d and -7f than other tissues.

Conclusion: While the expression of Lin28 protein and let-7 miRNA did not vary significantly with labour onset and delivery, changes in their bioactivity and impact on nuclear signalling pathways in human gestational tissues remain to be established.
Keyword Amnion
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2014 Collection
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Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 9 times in Scopus Article | Citations
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Created: Thu, 11 Apr 2013, 14:58:09 EST by Clare Tarlington on behalf of UQ Centre for Clinical Research