Epigenomic consequences of immortalized plant cell suspension culture

Tanurdzic, Milos, Vaughn, Matthew W., Jiang, Hongmei, Lee, Tae-Jin, Slotkin, R. Keith, Sosinski, Bryon, Thompson, William F., Doerge, R. W. and Martienssen, Robert A. (2008) Epigenomic consequences of immortalized plant cell suspension culture. PLoS Biology, 6 12: 2880-2895. doi:10.1371/journal.pbio.0060302


Author Tanurdzic, Milos
Vaughn, Matthew W.
Jiang, Hongmei
Lee, Tae-Jin
Slotkin, R. Keith
Sosinski, Bryon
Thompson, William F.
Doerge, R. W.
Martienssen, Robert A.
Title Epigenomic consequences of immortalized plant cell suspension culture
Journal name PLoS Biology   Check publisher's open access policy
ISSN 1544-9173
1545-7885
Publication date 2008-12
Sub-type Article (original research)
DOI 10.1371/journal.pbio.0060302
Open Access Status DOI
Volume 6
Issue 12
Start page 2880
End page 2895
Total pages 16
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Language eng
Abstract Plant cells grown in culture exhibit genetic and epigenetic instability. Using a combination of chromatin immunoprecipitation and DNA methylation profiling on tiling microarrays, we have mapped the location and abundance of histone and DNA modifications in a continuously proliferating, dedifferentiated cell suspension culture of Arabidopsis. We have found that euchromatin becomes hypermethylated in culture and that a small percentage of the hypermethylated genes become associated with heterochromatic marks. In contrast, the heterochromatin undergoes dramatic and very precise DNA hypomethylation with transcriptional activation of specific transposable elements (TEs) in culture. High throughput sequencing of small interfering RNA (siRNA) revealed that TEs activated in culture have increased levels of 21-nucleotide (nt) siRNA, sometimes at the expense of the 24-nt siRNA class. In contrast, TEs that remain silent, which match the predominant 24-nt siRNA class, do not change significantly in their siRNA profiles. These results implicate RNA interference and chromatin modification in epigenetic restructuring of the genome following the activation of TEs in immortalized cell culture.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biological Sciences Publications
 
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Created: Mon, 08 Apr 2013, 22:06:00 EST by Gail Walter on behalf of School of Biological Sciences