ABAP1 is a novel plant Armadillo BTB protein involved in DNA replication and transcription

Masuda, Hana Paula, Cabral, Luiz Mors, De Veylder, Lieven, Tanurdzic, Milos, Engler, Janice de Almeida, Geelen, Danny, Inze, Dirk, Martienssen, Robert A., Ferreira, Paulo C. G. and Hemerly, Adriana S. (2008) ABAP1 is a novel plant Armadillo BTB protein involved in DNA replication and transcription. EMBO Journal, 27 20: 2746-2756. doi:10.1038/emboj.2008.191


Author Masuda, Hana Paula
Cabral, Luiz Mors
De Veylder, Lieven
Tanurdzic, Milos
Engler, Janice de Almeida
Geelen, Danny
Inze, Dirk
Martienssen, Robert A.
Ferreira, Paulo C. G.
Hemerly, Adriana S.
Title ABAP1 is a novel plant Armadillo BTB protein involved in DNA replication and transcription
Journal name EMBO Journal   Check publisher's open access policy
ISSN 0261-4189
1460-2075
Publication date 2008-10
Sub-type Article (original research)
DOI 10.1038/emboj.2008.191
Volume 27
Issue 20
Start page 2746
End page 2756
Total pages 11
Place of publication London, United Kingdom
Publisher Nature Publishing
Language eng
Abstract In multicellular organisms, organogenesis requires a tight control of the balance between cell division and cell differentiation. Distinct signalling pathways that connect both cellular processes with developmental cues might have evolved to suit different developmental plans. Here, we identified and characterized a novel protein that interacts with pre-replication complex (pre-RC) subunits, designated Armadillo BTB Arabidopsis protein 1 (ABAP1). Overexpression of ABAP1 in plants limited mitotic DNA replication and decreased cell proliferation in leaves, whereas ABAP1 downregulation increased cell division rates. Activity of ABAP1 in transcription was supported by its association with the transcription factor AtTCP24. The ABAP1-AtTCP24 complex bound specifically to the promoters of AtCDT1a and AtCDT1b in vitro and in vivo. Moreover, expression levels of AtCDT1a and AtCDT1b were reduced in ABAP1-overexpressing plants and they were increased in plants with reduced levels of ABAP1. We propose that ABAP1 participates in a negative feedback loop regulating mitotic DNA replication during leaf development, either by repressing transcription of pre-RC genes and possibly by regulating pre-RC utilization through direct association with pre-RC components.
Keyword DNA replication
Gene transcription
Plant development
pre-RC
TCP
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biological Sciences Publications
 
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Created: Mon, 08 Apr 2013, 22:05:16 EST by Gail Walter on behalf of Examinations