A technique for obtaining monokaryotic haploid hyphae of Ustilago tritici, causal agent of wheat loose smut

Sharifnabi, B., Mitter, Neena and Sarbhoy, A. K. (2000) A technique for obtaining monokaryotic haploid hyphae of Ustilago tritici, causal agent of wheat loose smut. Indian Phytopathology, 53 1: 100-101.

Author Sharifnabi, B.
Mitter, Neena
Sarbhoy, A. K.
Title A technique for obtaining monokaryotic haploid hyphae of Ustilago tritici, causal agent of wheat loose smut
Formatted title
A technique for obtaining monokaryotic haploid hyphae of Ustilago tritici, causal agent of wheat loose smut
Journal name Indian Phytopathology   Check publisher's open access policy
ISSN 0367-973X
Publication date 2000
Sub-type Article (original research)
Volume 53
Issue 1
Start page 100
End page 101
Total pages 2
Place of publication New Delhi, India
Publisher Indian Phytopathological Society
Language eng
Abstract Twenty isolates of Ustilago tritici [U. segetum var. tritici] were collected from various parts of northern India and used for monokaryotic haplont production. Teliospores from the infected wheat samples were surface sterilized and 150-250 mul of sterilized teliospore suspension was evenly spread on Petri dishes containing 1.5-2 mm thick, 1.5% water agar and DL-aspartic acid (0.147 mg/ml water). The Petri dishes were incubated at 20℃ for about 30 hours. Dikaryon formation was observed and subsequently 1 cm2 blocks of medium from these plates were transferred to plates of 1/5 normal nutrient concentration of potato dextrose agar and incubated in the refrigerator overnight. The squares were then transferred to another layer of 1/5 PDA pre-warmed to25?and kept at this temperature for 4-6 hours. Monokaryotic haploid hyphae were obtained and isolated by microsurgery with very thin Pasteur pipettes and transferred to a thick layer of 1/5 PDA and kept at 20? Growth of haplonts was relatively slow and was visible only after 4-5 days. After 2 weeks, the verrucose colonies of fungus which were dense, cream to pinkish cream, could be seen. The mycelial mass production of the mycelium of haploid hyphae can be obtained by inoculation of potato sucrose broth in shaker incubation at 130 rpm, 20?for 14-20 days. This technique was effective in obtaining monokaryotic haploid hyphae for all 20 isolates of U. segetum var. tritici.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Alliance for Agriculture and Food Innovation
 
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Created: Mon, 08 Apr 2013, 11:19:51 EST by Dr Neena Mitter on behalf of Qld Alliance for Agriculture and Food Innovation