Diversity of heterotrimeric G-protein subunits in plants

Trusov, Yuri, Chakravorty, David and Botella, Jose Ramon (2012) Diversity of heterotrimeric G-protein subunits in plants. BMC Research Notes, 5 608.1-608.9. doi:10.1186/1756-0500-5-608

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Author Trusov, Yuri
Chakravorty, David
Botella, Jose Ramon
Title Diversity of heterotrimeric G-protein subunits in plants
Formatted title
Diversity of heterotrimeric G-protein γ subunits in plants
Journal name BMC Research Notes   Check publisher's open access policy
ISSN 1756-0500
Publication date 2012
Sub-type Article (original research)
DOI 10.1186/1756-0500-5-608
Open Access Status DOI
Volume 5
Start page 608.1
End page 608.9
Total pages 9
Place of publication London, United Kingdom
Publisher BioMed Central
Collection year 2013
Language eng
Formatted abstract
Background: Heterotrimeric G-proteins, consisting of three subunits Gα, Gβ and Gγ are present in most eukaryotes and mediate signaling in numerous biological processes. In plants, Gγ subunits were shown to provide functional selectivity to G-proteins. Three unconventional G subunits were recently reported in Arabidopsis, rice and soybean but no structural analysis has been reported so far. Their relationship with conventional Gγ subunits and taxonomical distribution has not been yet demonstrated.

Results: After an extensive similarity search through plant genomes, transcriptomes and proteomes we assembled over 200 non-redundant proteins related to the known Gγ subunits. Structural analysis of these sequences revealed that most of them lack the obligatory C-terminal prenylation motif (CaaX). According to their C-terminal structures we classified the plant Gγ subunits into three distinct types. Type A consists of G subunits with a putative prenylation motif. Type B subunits lack a prenylation motif and do not have any cysteine residues in the C-terminal region, while type C subunits contain an extended C-terminal domain highly enriched with cysteines. Comparative analysis of C-terminal domains of the proteins, intron-exon arrangement of the corresponding genes and phylogenetic studies suggested a common origin of all plant Gγ subunits.

Conclusion: Phylogenetic analyses suggest that types C and B most probably originated independently from type A ancestors. We speculate on a potential mechanism used by those Gγ subunits lacking isoprenylation motifs to anchor the Gβγ dimer to the plasma membrane and propose a new flexible nomenclature for plant Gγ subunits. Finally, in the light of our new classification, we give a word of caution about the interpretation of Gγ research in Arabidopsis and its generalization to other plant species.
Keyword Heterotrimeric G-proteins
Signal transduction
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2013 Collection
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Created: Mon, 08 Apr 2013, 11:15:24 EST by Professor Jimmy Botella on behalf of Qld Alliance for Agriculture and Food Innovation