West Nile virus NS2A protein facilitates virus-induced apoptosis independently of interferon response

Melian, Ezequiel Balmori, Edmonds, Judith H., Nagasaki, Tomoko Kim, Hinzman, Edward, Floden, Nadia and Khromykh, Alexander A. (2013) West Nile virus NS2A protein facilitates virus-induced apoptosis independently of interferon response. Journal of General Virology, 94 Part 2: 308-313. doi:10.1099/vir.0.047076-0


Author Melian, Ezequiel Balmori
Edmonds, Judith H.
Nagasaki, Tomoko Kim
Hinzman, Edward
Floden, Nadia
Khromykh, Alexander A.
Title West Nile virus NS2A protein facilitates virus-induced apoptosis independently of interferon response
Journal name Journal of General Virology   Check publisher's open access policy
ISSN 0022-1317
1465-2099
Publication date 2013-02
Sub-type Article (original research)
DOI 10.1099/vir.0.047076-0
Volume 94
Issue Part 2
Start page 308
End page 313
Total pages 6
Place of publication Reading, Berks, United Kingdom
Publisher Society for General Microbiology
Collection year 2014
Language eng
Abstract The flavivirus NS2A protein is a small, multifunctional protein, involved in replication, virion formation and regulation of the innate immune response. Using the Kunjin strain of West Nile virus (WNVKUN) we previously demonstrated that a single amino acid change from alanine to proline at position 30 of the NS2A protein (A30P) reduced viral cytopathicity in cells and virulence in mice. To further investigate functions of the NS2A protein we have substituted alanine at position 30 with different amino acids (A30 mutants) in a WNVKUN infectious clone. The virulence of mutant viruses in wild-type (WT) and IRF3/IRF7 double-knockout mice was influenced by the amino acid change and ranged from high to low in the order of WT>A30L>A30E>A30P/A30G. Moreover, infection of beta interferon (IFN-β)-deficient Vero cells with A30P virus showed less pronounced chromosomal DNA degradation and lower percentage of cells with positive TUNEL labelling than in WT virus infection, indicating a role for the WT NS2A protein in IFN-independent apoptotic cell death.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Chemistry and Molecular Biosciences
 
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Created: Fri, 05 Apr 2013, 13:19:07 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences