Sperm chromatin in beef bulls in tropical environments

D'Occhio, Michael J., Hengstberger, Kirsten J., Tutt, Desmond, Holroyd, Richard G., Fordyce, Geoffry, Boe-Hansen, Gry B. and Johnston, Steve D. (2013) Sperm chromatin in beef bulls in tropical environments. Theriogenology, 79 6: 946-952. doi:10.1016/j.theriogenology.2013.01.012


Author D'Occhio, Michael J.
Hengstberger, Kirsten J.
Tutt, Desmond
Holroyd, Richard G.
Fordyce, Geoffry
Boe-Hansen, Gry B.
Johnston, Steve D.
Title Sperm chromatin in beef bulls in tropical environments
Journal name Theriogenology   Check publisher's open access policy
ISSN 0093-691X
1879-3231
Publication date 2013-04-01
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.theriogenology.2013.01.012
Volume 79
Issue 6
Start page 946
End page 952
Total pages 7
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Collection year 2014
Language eng
Abstract Sperm chromatin statuswas assessed in 565 Zebu and Zebu crossbred beef bulls in extensive tropical environments using the sperm chromatin structure assay (SCSA). The SCSA involved exposure of sperm to acid hydrolysis for 0.5 or 5.0 minutes, followed by flow cytometry to ascertain relative amounts of double-stranded (normal) and single-stranded (denatured)DNA, which was used to generate a DNA fragmentation index (%DFI). With conventional SCSA (0.5-minute SCSA), 513 bulls (91%) had<15 %DFI, 24 bulls (4%) had 15 to 27 %DFI, and 28 bulls (5%) had >27 %DFI. In 5.0-minute SCSA, 432 bulls (76%) had <15 %DFI, 68 bulls (12%)had 15 to 27 %DFI and 65 bulls (12%) had >27 %DFI. For most bulls, the SCSAwas repeatable on two to four occasions; however, because most bulls had <15 %DFI, repeatability of the SCSAwill need to be determined in a largernumber of bulls in the 15 to 27 %DFI and>27 %DFI categories. The %DFI was negatively correlated with several bull semen parameters and the strongest negative correlation was with normal sperm. There was a strong positive correlation between %DFI and sperm head abnormalities. Based on these findings, most Zebu beef bulls in extensive tropical environments had relatively stable sperm chromatin. Based on the apparent negative correlations with conventional semen parameters, we inferred that the SCSA measured a unique feature of sperm quality, which has also been suggested for other species. Further studies on the relationships between sperm chromatin stability and fertility are required in beef bulls before chromatin status can be used as an additional predictor of the siring capacity of individual bulls in extensive multiple-sire herds.
Keyword Bull
Sperm chromatin
Semen parameter
Sperm chromatin structure assay
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Thu, 04 Apr 2013, 14:26:02 EST by Dr Gry Boe-hansen on behalf of School of Agriculture and Food Sciences