Sucrose mobilisation in sugarcane stalk induced by heterotrophic axillary bud growth

O’Neill, Brian P., Purnell, Matthew P., Anderson, David J., Nielsen, Lars K. and Brumbley, Stevens M. (2012) Sucrose mobilisation in sugarcane stalk induced by heterotrophic axillary bud growth. Tropical Plant Biology, 5 2: 173-182. doi:10.1007/s12042-012-9097-6

Author O’Neill, Brian P.
Purnell, Matthew P.
Anderson, David J.
Nielsen, Lars K.
Brumbley, Stevens M.
Title Sucrose mobilisation in sugarcane stalk induced by heterotrophic axillary bud growth
Journal name Tropical Plant Biology   Check publisher's open access policy
ISSN 1935-9756
Publication date 2012-06-01
Sub-type Article (original research)
DOI 10.1007/s12042-012-9097-6
Open Access Status
Volume 5
Issue 2
Start page 173
End page 182
Total pages 10
Place of publication New York, United States
Publisher Springer New York LLC
Collection year 2013
Language eng
Formatted abstract
A theoretical high-yield sugarcane biofactory can be idealised as containing culm tissue that functions as a secondary source tissue rather than a sink. To investigate this potential process, heterotrophic axillary bud outgrowth from sugarcane (Saccharum spp. hybrids) setts was used as a model system to demonstrate that sucrose is a mobilisable carbon source. The outgrowth and subsequent biomass accumulation of axillary buds from two-eye setts of mature sugarcane stalks grown in the dark was used to measure carbon mobilisation from sett internode pith tissue. After 42 days growth 99.0 ± 0.72% of sett internode pith sucrose was depleted and 2. 66 ± 0.16 g of new tissue accumulated. Comparison with a control treatment in which axillary buds were excised at day zero demonstrated that carbon mobilisation was driven by the accumulation of new biomass. Profiling of soluble carbohydrates (viz. sucrose, glucose and fructose), starch, total soluble protein, total amino nitrogen, free amino acids and total insoluble material showed that the sucrose stored in the sett internode pith was the only available carbon source of sufficient size at day zero for the observed biomass accumulation. Other metabolites mobilised were glucose, fructose and some amino acids, notably isoleucine and leucine that were depleted in shoot treatment setts at day 42.
Keyword Carbon partitioning
Carbon source sink
Sucrose derivatives
Sucrose mobilisation
Sugarcane biofactory
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
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Citation counts: TR Web of Science Citation Count  Cited 2 times in Thomson Reuters Web of Science Article | Citations
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Created: Fri, 29 Mar 2013, 03:24:12 EST by Lars Nielsen on behalf of Aust Institute for Bioengineering & Nanotechnology