The impact of saliva collection and processing methods on CRP, IgE, and Myoglobin immunoassays

Mohamed, Roslinda, Campbell, Jennifer-Leigh, Cooper-White, Justin, Dimeski, Goce and Punyadeera, Chamindie (2012) The impact of saliva collection and processing methods on CRP, IgE, and Myoglobin immunoassays. Clinical and Translational Medicine, 1 19.1-19.7. doi:10.1186/2001-1326-1-19

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Author Mohamed, Roslinda
Campbell, Jennifer-Leigh
Cooper-White, Justin
Dimeski, Goce
Punyadeera, Chamindie
Total Author Count Override 5
Title The impact of saliva collection and processing methods on CRP, IgE, and Myoglobin immunoassays
Journal name Clinical and Translational Medicine   Check publisher's open access policy
ISSN 2001-1326
Publication date 2012-12-02
Year available 2012
Sub-type Article (original research)
DOI 10.1186/2001-1326-1-19
Open Access Status DOI
Volume 1
Start page 19.1
End page 19.7
Total pages 8
Place of publication New York , NY, United States
Publisher Springer
Collection year 2013
Language eng
Formatted abstract
Background: Owing to its ease of collection, saliva is potentially the sample of choice in diagnosis. Salivary biomolecules have provided a porthole in surveying a person’s health and well-being. Our study aims were (1) to demonstrate the effects of pre-analytical steps, collection and pre-processing techniques on salivary protein
detection and (2) to establish an indication of salivary reference intervals for 3 biomolecules of clinical interest.

Methods: Saliva samples were collected from participants (n = 25, ages 20–35 years) using the following methods: no stimulation (resting/unstimulated), mechanical, and acid stimulation. The saliva was prepared for analysis by: unprocessed, post standard centrifugation in a container without any additives, and centrifugation using Centrifugal Filter Unit (Amicon® Ultra-0.5). AlphaLisa® assays were used to measure the levels of C-Reactive Protein (CRP), Immunoglobin (IgE) and myoglobin in saliva samples.

Results: Saliva flow rates were lowest with the resting/drooling collection method. The lowest total protein concentration was with acid stimulation. Unstimulated and mechanically stimulated collections produced no effect on the CRP and IgE levels while myoglobin levels were highest with the unstimulated collection. Acid stimulation
had a negative impact on the measured concentrations of IgE and myoglobin (except for CRP levels).

Conclusion: Mechanical stimulation was the most viable option for collecting saliva without affecting the levels of CRP and myoglobin. The processing methods had an adverse effect on the concentration of total protein as well as on CRP and IgE concentrations.
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Created: Tue, 26 Mar 2013, 14:34:46 EST by Dr Chamindie Punyadeera on behalf of School of Biomedical Sciences