Pineapple translation factor SUI1 and ribosomal protein L36 promoters drive constitutive transgene expression patterns in Arabidopsis thaliana

Koia, Jonni, Moyle, Richard, Hendry, Caroline, Lim, Lionel and Botella, Jose Ramon (2013) Pineapple translation factor SUI1 and ribosomal protein L36 promoters drive constitutive transgene expression patterns in Arabidopsis thaliana. Plant Molecular Biology, 81 4-5: 327-336. doi:10.1007/s11103-012-0002-3


Author Koia, Jonni
Moyle, Richard
Hendry, Caroline
Lim, Lionel
Botella, Jose Ramon
Title Pineapple translation factor SUI1 and ribosomal protein L36 promoters drive constitutive transgene expression patterns in Arabidopsis thaliana
Formatted title
Pineapple translation factor SUI1 and ribosomal protein L36 promoters drive constitutive transgene expression patterns in Arabidopsis thaliana
Journal name Plant Molecular Biology   Check publisher's open access policy
ISSN 0167-4412
1573-5028
Publication date 2013-03
Year available 2012
Sub-type Article (original research)
DOI 10.1007/s11103-012-0002-3
Volume 81
Issue 4-5
Start page 327
End page 336
Total pages 10
Place of publication Dordrecht, Netherlands
Publisher Springer
Collection year 2013
Language eng
Formatted abstract
The availability of a variety of promoter sequences is necessary for the genetic engineering of plants, in basic research studies and for the development of transgenic crops. In this study, the promoter and 5′ untranslated regions of the evolutionally conserved protein translation factor SUI1 gene and ribosomal protein L36 gene were isolated from pineapple and sequenced. Each promoter was translationally fused to the GUS reporter gene and transformed into the heterologous plant system Arabidopsis thaliana. Both the pineapple SUI1 and L36 promoters drove GUS expression in all tissues of Arabidopsis at levels comparable to the CaMV35S promoter. Transient assays determined that the pineapple SUI1 promoter also drove GUS expression in a variety of climacteric and non-climacteric fruit species. Thus the pineapple SUI1 and L36 promoters demonstrate the potential for using translation factor and ribosomal protein genes as a source of promoter sequences that can drive constitutive transgene expression patterns.
Keyword Ribosomal protein L36
Sui1
Promoter
Constitutive
Gus
Pineapple
Arabidopsis
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online 22 December 2012.

 
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