Calcium channels, pumps and channel modulators and proliferation of SKBR3 HER2-positive cancer cells

Pera, E., Peters, A. A., Roberts-Thomson, S. J. and Monteith, G. R. (2012). Calcium channels, pumps and channel modulators and proliferation of SKBR3 HER2-positive cancer cells. In: Programme, Abstracts, List of Participants. 12th Meeting of the European Calcium Society (ECS 2012), Toulouse, France, (164-164). 9-12 September 2012.

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Name Description MIMEType Size Downloads
Author Pera, E.
Peters, A. A.
Roberts-Thomson, S. J.
Monteith, G. R.
Title of paper Calcium channels, pumps and channel modulators and proliferation of SKBR3 HER2-positive cancer cells
Conference name 12th Meeting of the European Calcium Society (ECS 2012)
Conference location Toulouse, France
Conference dates 9-12 September 2012
Proceedings title Programme, Abstracts, List of Participants
Publication Year 2012
Sub-type Published abstract
Start page 164
End page 164
Total pages 1
Language eng
Formatted Abstract/Summary
HER2-positive breast tumors represent approximately 18-20% of all breast cancers and are characterized by an overexpression of the growth factor receptor HER2. The role of calcium channels and pumps has been linked to several types of cancers, including breast cancer, and their altered expression may contribute to the development and progression of some breast tumors. However, the expression of these transporters and the consequences of their inhibition have not been fully evaluated in HER2-positve breast cancer cells such as the SKBR3 cell line. Using siRNA and high content imaging technologies, the effect of silencing of 16 calcium channels, pumps and calcium modulators on SKBR3 cell proliferation was evaluated using EdU-Alexa Fluor® 555 staining (Life Technologies). The silencing of the transporters and modulators was confirmed using real time RT-PCR. Assessment of proliferation 6 days after siRNA treatment suggests that stromal interaction molecule 1 (STIM1) and two pore channel (TPC2) silencing significantly decreases the proliferation of SKBR3 cells. STIM1 showed a higher mRNA level expression compared to its related isoform STIM2; whereas TPC2 mRNA levels were lower compared to TPC1. Relatively high levels of the plasma membrane calcium ATPase (PMCA) isoforms 1 and 4, as well as secretory pathway calcium-ATPase (SPCA) isoforms 1 and 2 and the transient receptor potential (TRP) cation channel TRPV6 were present in SKBR3 cells, but silencing of these channels and pumps did not affect the proliferation of SKBR3 cells.

These studies suggest that STIM1 and TPC2 silencing decreases the proliferation of SKBR3 cells, thus these two proteins may be involved in tumor progression in HER2-positive breast cancers and should be the focus of further investigation.

Keyword HER2
Breat cancer
Profiferaton
Q-Index Code EX
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Session VI - Calcium Development and Stem Cells.

Document type: Conference Paper
Collection: School of Pharmacy Publications
 
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Created: Fri, 22 Mar 2013, 13:57:41 EST by Myrtle Sahabandu on behalf of School of Pharmacy