Novel members of the family Pasteurellaceae are common in kangaroos

Bisgaard, Magnes, Blackall, Pat J. and Christensen, Henrik (2012). Novel members of the family Pasteurellaceae are common in kangaroos. In: IVVDC 2012: 6th International Veterinary Vaccines and Diagnostics Conference, Cairns, Australia, (). 29 July - 1 August 2012.

Author Bisgaard, Magnes
Blackall, Pat J.
Christensen, Henrik
Title of paper Novel members of the family Pasteurellaceae are common in kangaroos
Formatted title
Novel members of the family Pasteurellaceae are common in kangaroos
Conference name IVVDC 2012: 6th International Veterinary Vaccines and Diagnostics Conference
Conference location Cairns, Australia
Conference dates 29 July - 1 August 2012
Publication Year 2012
Sub-type Oral presentation
Language eng
Formatted Abstract/Summary
No specific reports exist on the presence of members of the family Pasteurellaceae in macropods. This initial investigation examined free wild kangaroos for the presence of Pasteurellaceae like bacteria and then undertook initial characterization tests. Â A total of 98 isolates of Pasteurellaceae were obtained from oropharyngeal swabs collected from legally harvested wild kangaroos in southern Queensland. The 98 isolates were characterized by partial rpoB sequence analysis and formed 3 groups (55 isolates in Group 1, 21 in Group 2 and 22 in Group 3). 16S rRNA sequencing of 18 selected strains showed a monophyletic but diverse group (>94.8% within group similarity) which appear to represent novel genera and species. Existing close relatives were as follows:- Group 1 - Actinobacillus lignieresii (95.6%) and Mannheimia caviae (95.5%); Group 2 - A. lignieresii (95.6%) and [A.] minor (96.6%); Group 3 - A. arthritidis (95.4%). Phenotypical characterization (32 tests) showed that 30 selected strains from the 3 groups were Gram negative, oxidase and nitrate positive and catalase negative. Satellitism was variable between within the groups. Separation of the 3 groups was not fully achieved. Some of the isolates demonstrated a high level V-factor requirement and special methods were required for the characterization work.
Q-Index Code EX
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Conference Paper
Collection: Queensland Alliance for Agriculture and Food Innovation
 
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Created: Mon, 18 Mar 2013, 20:33:48 EST by Dr Patrick Blackall on behalf of Centre for Animal Science