Myosin VI small insert isoform maintains exocytosis by tethering secretory granules to the cortical actin

Tomatis, Vanesa M., Papadopulos, Andreas, Malintan, Nancy T., Martin, Sally, Wallis, Tristan, Gormal, Rachel S., Kendrick-Jones, John, Buss, Folma and Meunier, Frederic A. (2013) Myosin VI small insert isoform maintains exocytosis by tethering secretory granules to the cortical actin. Journal of Cell Biology, 200 3: 301-320. doi:10.1083/jcb.201204092

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Author Tomatis, Vanesa M.
Papadopulos, Andreas
Malintan, Nancy T.
Martin, Sally
Wallis, Tristan
Gormal, Rachel S.
Kendrick-Jones, John
Buss, Folma
Meunier, Frederic A.
Title Myosin VI small insert isoform maintains exocytosis by tethering secretory granules to the cortical actin
Journal name Journal of Cell Biology   Check publisher's open access policy
ISSN 0021-9525
1540-8140
Publication date 2013-02
Sub-type Article (original research)
DOI 10.1083/jcb.201204092
Open Access Status File (Publisher version)
Volume 200
Issue 3
Start page 301
End page 320
Total pages 20
Place of publication New York, NY, United States
Publisher Rockefeller University Press
Collection year 2014
Language eng
Abstract Before undergoing neuroexocytosis, secretory granules (SGs) are mobilized and tethered to the cortical actin network by an unknown mechanism. Using an SG pull-down assay and mass spectrometry, we found that myosin VI was recruited to SGs in a Ca2+- dependent manner. Interfering with myosin VI function in PC12 cells reduced the density of SGs near the plasma membrane without affecting their biogenesis. Myosin VI knockdown selectively impaired a late phase of exocytosis, consistent with a replenishment defect. This exocytic defect was selectively rescued by expression of the myosin VI small insert (SI) isoform, which efficiently tethered SGs to the cortical actin network. These myosin VI SI-specific effects were prevented by deletion of a c-Src kinase phosphorylation DYD motif, identified in silico. Myosin VI SI thus recruits SGs to the cortical actin network, potentially via c-Src phosphorylation, thereby maintaining an active pool of SGs near the plasma membrane.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Brain Institute Publications
Official 2014 Collection
 
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