Expression of Survivin and Caspase 3 in Oral Squamous Cell Carcinoma and Peritumoral Tissue

Li, S. X., Chai, L., Cai, Z. G., Jin, L. J., Chen, Y., Wu, H. R. and Sun, Z. (2012) Expression of Survivin and Caspase 3 in Oral Squamous Cell Carcinoma and Peritumoral Tissue. Asian Pacific Journal of Cancer Prevention, 13 10: 5027-5031. doi:10.7314/APJCP.2012.13.10.5027


Author Li, S. X.
Chai, L.
Cai, Z. G.
Jin, L. J.
Chen, Y.
Wu, H. R.
Sun, Z.
Title Expression of Survivin and Caspase 3 in Oral Squamous Cell Carcinoma and Peritumoral Tissue
Journal name Asian Pacific Journal of Cancer Prevention   Check publisher's open access policy
ISSN 1513-7368
Publication date 2012
Year available 2012
Sub-type Article (original research)
DOI 10.7314/APJCP.2012.13.10.5027
Volume 13
Issue 10
Start page 5027
End page 5031
Total pages 5
Place of publication Rajchathewee, Bangkok, Thailand
Publisher Asian Pacific Organization for Cancer Prevention
Collection year 2013
Language eng
Formatted abstract
This study was conducted to investigate the expression of survivin and caspase 3 in oral squamous cell carcinoma and peritumoral tissue, and possible pathogenesis mechanisms. We used ELISA and western blotting to detect the protein expression levels of survivin and caspase 3 in tissue. In situ hybridization and real-time PCR were applied to assess mRNA expression levels. In this study, 13 tumor samples and 13 peritumoral tissue samples were collected from oral squamous cell carcinoma patients and 10 normal tissue samples obtained from patients without tumor. The result showed that the protein and mRNA expression of survivin in carcinoma was the highest among three types of tissue; following was that in peritumoral tissue. No difference in caspase 3 zymogen between peritumoral tissue and normal tissue could be found, while it was evidently decreased in carcinoma tissue. Activated caspase 3 was detected in normal tissue but could not be identified in peritumoral or carcinoma tissue. Our results indicate that the expression of survivin is apparently elevated in tumoral and peritumoral tissue. Expression of activated caspase 3 was not detected in tumoral tissue and the expression of caspase 3 zymogen was decreased in tumoral tissue. Our findings suggest that survivin may inhibit both synthesis and activation of caspase 3, hence inhibiting cell apoptosis and facililitating eventual development of oral squamous cell carcinoma.
Keyword Survivin
Caspase 3
Oral squamous cell carcinoma
Apoptosis
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Dentistry Publications
 
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