Conservation of coconut (cocos nucifera l.) germplasm at sub-zero temperature

Sisunandar, Sopade, Peter A., Samosir, Yohannes M. S., Rival, Alain and Adkins, Steve W. (2012) Conservation of coconut (cocos nucifera l.) germplasm at sub-zero temperature. Cryoletters, 33 6: 465-475.

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Name Description MIMEType Size Downloads
Author Sisunandar
Sopade, Peter A.
Samosir, Yohannes M. S.
Rival, Alain
Adkins, Steve W.
Total Author Count Override 5
Title Conservation of coconut (cocos nucifera l.) germplasm at sub-zero temperature
Formatted title
Conservation of coconut (cocos nucifera l.) germplasm at sub-zero temperature
Journal name Cryoletters   Check publisher's open access policy
ISSN 0143-2044
1742-0644
Publication date 2012-11
Sub-type Article (original research)
Volume 33
Issue 6
Start page 465
End page 475
Total pages 11
Place of publication Great Marlings, United Kingdom
Publisher CryoLetters
Collection year 2013
Language eng
Formatted abstract
Protocols are proposed for the low (-20°C) and ultra-low (-80°C) temperature storage of coconut (Cocos nucifera L.) embryos. A tissue dehydration step prior to storage, and a rapid warming step upon recovery optimized the protocol. The thermal properties of water located within the embryos were monitored using differential scanning calorimetry (DSC). In the most efficient version of the protocol, embryos were dehydrated under a sterile air flow in a dehydration solution containing glucose (3.33 M) and glycerol (15%) for16 hours. This protocol decreased the embryo water content from 77 to 29% FW and at the same time reduced the amount of freezable water down to 0.03%. The dehydrated embryos could be stored for up to 3 weeks at -20°C (12 % producing normal plants upon recovery) or 26 weeks at -80°C (28% producing normal plants upon recovery). These results indicate that it is possible to store coconut germplasm on a medium term basis using an ultra-deep freezer unit. However for more efficient, long term storage, cryopreservation remains the preferred option.
Keyword Dehydration
Cryopreservation
Differential scanning calorimetry
Embryo culture
Medium-term conservation
Recalcitrant seeds
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2013 Collection
Centre for Nutrition and Food Sciences Publications
 
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