Microarray analysis of gene expression profiles in ripening pineapple fruits

Koia, Jonni H., Moyle, Richard L. and Botella, Jose R. (2012) Microarray analysis of gene expression profiles in ripening pineapple fruits. BMC Plant Biology, 12 240.1-240.13. doi:10.1186/1471-2229-12-240


Author Koia, Jonni H.
Moyle, Richard L.
Botella, Jose R.
Title Microarray analysis of gene expression profiles in ripening pineapple fruits
Journal name BMC Plant Biology   Check publisher's open access policy
ISSN 1471-2229
Publication date 2012-12
Year available 2012
Sub-type Article (original research)
DOI 10.1186/1471-2229-12-240
Open Access Status DOI
Volume 12
Start page 240.1
End page 240.13
Total pages 13
Place of publication London, United Kingdom
Publisher BioMed Central
Collection year 2013
Language eng
Formatted abstract
Background: Pineapple (Ananas comosus) is a tropical fruit crop of significant commercial importance. Although the physiological changes that occur during pineapple fruit development have been well characterized, little is known about the molecular events that occur during the fruit ripening process. Understanding the molecular basis of pineapple fruit ripening will aid the development of new varieties via molecular breeding or genetic modification. In this study we developed a 9277 element pineapple microarray and used it to profile gene expression changes that occur during pineapple fruit ripening.

Results: Microarray analyses identified 271 unique cDNAs differentially expressed at least 1.5-fold between the mature green and mature yellow stages of pineapple fruit ripening. Among these 271 sequences, 184 share significant homology with genes encoding proteins of known function, 53 share homology with genes encoding proteins of unknown function and 34 share no significant homology with any database accession. Of the 237 pineapple sequences with homologs, 160 were up-regulated and 77 were down-regulated during pineapple fruit ripening. DAVID Functional Annotation Cluster (FAC) analysis of all 237 sequences with homologs revealed confident enrichment scores for redox activity, organic acid metabolism, metalloenzyme activity, glycolysis, vitamin C biosynthesis, antioxidant activity and cysteine peptidase activity, indicating the functional significance and importance of these processes and pathways during pineapple fruit development. Quantitative real-time PCR analysis validated the microarray expression results for nine out of ten genes tested.

Conclusions: This is the first report of a microarray based gene expression study undertaken in pineapple. Our bioinformatic analyses of the transcript profiles have identified a number of genes, processes and pathways with putative involvement in the pineapple fruit ripening process. This study extends our knowledge of the molecular basis of pineapple fruit ripening and non-climacteric fruit ripening in general.
Keyword Pineapple
Non climacteric
Fruit ripening
Microarray
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Author supplied pagination 240-252

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2013 Collection
 
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