High throughput investigative dermatology in 2012 and beyond: a new era beckons

Gilmore, Stephen J. (2013) High throughput investigative dermatology in 2012 and beyond: a new era beckons. Australasian Journal of Dermatology, 54 1: 1-8. doi:10.1111/j.1440-0960.2012.00883.x


Author Gilmore, Stephen J.
Title High throughput investigative dermatology in 2012 and beyond: a new era beckons
Journal name Australasian Journal of Dermatology   Check publisher's open access policy
ISSN 0004-8380
1440-0960
Publication date 2013-02
Year available 2012
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1111/j.1440-0960.2012.00883.x
Volume 54
Issue 1
Start page 1
End page 8
Total pages 8
Place of publication Richmond, VIC, Australia
Publisher Wiley-Blackwell
Collection year 2013
Language eng
Abstract High throughput molecular biology began around the mid-1990s with the introduction of microarrays – a technology that enabled investigators to quantify the cellular expression levels of tens of thousands of mRNA transcripts simultaneously. To date, a large number of microarray experiments have been performed in the investigation of RNA expression signatures in normal and pathological tissues. This review focuses on a next generation tool in high throughput investigation: RNA sequencing or RNA-Seq, highlighting its advantages over traditional microarray investigation and discussing its utility in investigative dermatology. In contrast with the results obtained from microarray experiments, RNA-Seq generates mRNA abundance counts, can identify novel transcripts and splice variants, and provides sequence resolution at the level of single base-pairs. Implementing RNA-Seq in the investigation of skin disease will yield novel insights into the pathogenesis of disease, will facilitate the discovery of new diseases and new mechanisms of disease, and will allow researchers to probe genetic disease in high resolution and with unprecedented efficiency.
Keyword Dermatology
Microarrays
mRNA
RNA-Seq
RNA sequencing
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online 16 April 2012.

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: Official 2013 Collection
School of Medicine Publications
 
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