Complete chloroplast genome sequence of Magnolia grandiflora and comparative analysis with related species

Li, XiWen, Gao, HuanHuan, Wang, YiTao, Song, JingYuan, Henry, Robert, Wu, HeZhen, Hu, ZhiGang, Yao, Hui, Luo, HongMei, Luo, Kun, Pan, HongLin and Chen, ShiLin (2013) Complete chloroplast genome sequence of Magnolia grandiflora and comparative analysis with related species. Science China Life Sciences, 56 2: 189-198. doi:10.1007/s11427-012-4430-8


Author Li, XiWen
Gao, HuanHuan
Wang, YiTao
Song, JingYuan
Henry, Robert
Wu, HeZhen
Hu, ZhiGang
Yao, Hui
Luo, HongMei
Luo, Kun
Pan, HongLin
Chen, ShiLin
Title Complete chloroplast genome sequence of Magnolia grandiflora and comparative analysis with related species
Formatted title
Complete chloroplast genome sequence of Magnolia grandiflora and comparative analysis with related species
Journal name Science China Life Sciences   Check publisher's open access policy
ISSN 1674-7305
1869-1889
Publication date 2013-02
Year available 2013
Sub-type Article (original research)
DOI 10.1007/s11427-012-4430-8
Open Access Status DOI
Volume 56
Issue 2
Start page 189
End page 198
Total pages 10
Place of publication Beijing, China
Publisher Zhongguo Kexue Zazhishe
Collection year 2014
Language eng
Formatted abstract
Magnolia grandiflora is an important medicinal, ornamental and horticultural plant species. The chloroplast (cp) genome of M. grandiflora was sequenced using a 454 sequencing platform and the genome structure was compared with other related species. The complete cp genome of M. grandiflora was 159623 bp in length and contained a pair of inverted repeats (IR) of 26563 bp separated by large and small single copy (LSC, SSC) regions of 87757 and 18740 bp, respectively. A total of 129 genes were successfully annotated, 18 of which included introns. The identity, number and GC content of M. grandiflora cp genes were similar to those of other Magnoliaceae species genomes. Analysis revealed 218 simple sequence repeat (SSR) loci, most composed of A or T, contributing to a bias in base composition. The types and abundances of repeat units in Magnoliaceae species were relatively conserved and these loci will be useful for developing M. grandiflora cp genome vectors. In addition, results indicated that the cp genome size in Magnoliaceae species and the position of the IR border were closely related to the length of the ycf1 gene. Phylogenetic analyses based on 66 shared genes from 30 species using maximum parsimony (MP) and maximum likelihood (ML) methods provided strong support for the phylogenetic position of Magnolia. The availability of the complete cp genome sequence of M. grandiflora provides valuable information for breeding of desirable varieties, cp genetic engineering, developing useful molecular markers and phylogenetic analyses in Magnoliaceae.
Keyword Intron
Inverted repeats
SSR
Phylogenetics
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
Official 2014 Collection
 
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