Chronic interstitial fibrosis in the rat kidney induced by long-term (6-mo) exposure to lithium

Walker, Robert J., Leader, John P., Bedford, Jennifer J., Gobe, Glenda, Davis, Gerard, Vos, Frederiek E., deJong, Sylvia and Schollum, John B. W. (2013) Chronic interstitial fibrosis in the rat kidney induced by long-term (6-mo) exposure to lithium. American Journal of Physiology: Renal Physiology, 304 3: F300-F307. doi:10.1152/ajprenal.00182.2012


Author Walker, Robert J.
Leader, John P.
Bedford, Jennifer J.
Gobe, Glenda
Davis, Gerard
Vos, Frederiek E.
deJong, Sylvia
Schollum, John B. W.
Title Chronic interstitial fibrosis in the rat kidney induced by long-term (6-mo) exposure to lithium
Journal name American Journal of Physiology: Renal Physiology   Check publisher's open access policy
ISSN 1931-857X
Publication date 2013-02-01
Year available 2013
Sub-type Article (original research)
DOI 10.1152/ajprenal.00182.2012
Volume 304
Issue 3
Start page F300
End page F307
Total pages 8
Place of publication Bethesda, MD, United States
Publisher American Physiological Society
Collection year 2014
Language eng
Abstract There is a lack of suitable animal models that replicate the slowly progressive chronic interstitial fibrosis that is characteristic of many human chronic nephropathies. We describe a chronic long-term (6-mo) model of lithium-induced renal fibrosis, with minimal active inflammation, which mimics chronic kidney interstitial fibrosis seen in the human kidney. Rats received lithium via their chow (60 mmol lithium/kg food) daily for 6 mo. No animals died during the exposure. Nephrogenic diabetes insipidus was established by 3 wk and persisted for the 6 mo. Following metabolic studies, the animals were killed at 1, 3, and 6 mo and the kidneys were processed for histological and immunohistochemical studies. Progressive interstitial fibrosis, characterized by increasing numbers of myofibroblasts, enhanced transforming growth factor-β1 expression and interstitial collagen deposition, and a minimal inflammatory cellular response was evident. Elucidation of the underlying mechanisms of injury in this model will provide a greater understanding of chronic interstitial fibrosis and allow the development of intervention strategies to prevent injury.
Keyword lithium
fibrosis
chronic kidney disease
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Medicine Publications
 
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