Cold-induced precipitation of a monoclonal IgM: a negative activation enthalpy reaction

Meliga, Stefano C., Farrugia, William, Ramsland, Paul A. and Falconer, Robert J. (2013) Cold-induced precipitation of a monoclonal IgM: a negative activation enthalpy reaction. Journal of Physical Chemistry B, 117 2: 490-494. doi:10.1021/jp309109k

Author Meliga, Stefano C.
Farrugia, William
Ramsland, Paul A.
Falconer, Robert J.
Title Cold-induced precipitation of a monoclonal IgM: a negative activation enthalpy reaction
Journal name Journal of Physical Chemistry B   Check publisher's open access policy
ISSN 1520-6106
Publication date 2013-01-17
Year available 2012
Sub-type Article (original research)
DOI 10.1021/jp309109k
Volume 117
Issue 2
Start page 490
End page 494
Total pages 5
Place of publication Washington, DC, United States
Publisher American Chemical Society
Collection year 2013
Language eng
Abstract Cold-induced precipitation of a monoclonal IgM cryoglobulin isolated from a patient with Waldenström's macroglobulinemia was observed to have a negative activation enthalpy. The rate of the reaction increased, as the temperature decreased. Differential scanning calorimetry of the monoclonal IgM showed precipitation as an inverted peak during a downward temperature scan. The transition temperature was between 14 and 15 °C and was possibly concentration dependent. At temperatures below the transition the precipitation was best described by second-order kinetics. The difference in change in enthalpy between precipitation and disassociation suggests that cold-induced precipitation had a fast precipitation stage followed by a slower consolidation reaction. Negligible curvature of the Eyring plot suggested the precipitation reaction was dominated by van der Waal forces and hydrogen bonding. Conversely, during an upward temperature scan, disassociation was observed as a positive enthalpy peak. This reaction had two stages, a reaction undoing consolidation followed by heat-induced disassociation that had first-order kinetics.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published: 14 December 2012.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
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