Stable isotopic labeling for proteomics

Ashman, Keith, Ruppen Canas, Maria Isabel, Luque-Garcia, Jose L. and Garcia Martinez, Fernando (2011). Stable isotopic labeling for proteomics. In Alexander R. Ivanov and Alexander V. Lazarev (Ed.), Sample preparation in biological mass spectrometry (pp. 549-573) Dordrecht, Netherlands: Springer. doi:10.1007/978-94-007-0828-0_27


Author Ashman, Keith
Ruppen Canas, Maria Isabel
Luque-Garcia, Jose L.
Garcia Martinez, Fernando
Title of chapter Stable isotopic labeling for proteomics
Title of book Sample preparation in biological mass spectrometry
Place of Publication Dordrecht, Netherlands
Publisher Springer
Publication Year 2011
Sub-type Research book chapter (original research)
DOI 10.1007/978-94-007-0828-0_27
ISBN 9789400707580
9789400702820
Editor Alexander R. Ivanov
Alexander V. Lazarev
Chapter number 27
Start page 549
End page 573
Total pages 25
Total chapters 49
Language eng
Abstract/Summary Since the coining of the term Proteome, the field of Proteomics has developed rapidly and come to rely heavily on mass spectrometry, initially for protein identification but recently the use of stable isotopic labels for protein quantitation has grown in importance. This trend has been driven by improvements in the mass spectrometers and reagents but also by the need to understand the molecular dynamics of cells. Since proteins are important effector molecules in most biochemical processes, key questions to be answered are: how much is present and when. In this chapter we describe the various methods currently available to quantitate proteins based on stable isotope protein labeling and discuss their merits as well as some of the issues still to be addressed.
Keyword Proteomics
Quantitation
Stable isotope labeling
iTRAQ
SILAC
Fractionation
HPLC
Mass spectrometer
MALDI
Electrospray
Peptides
Amines
Protein
Derivatization
Biomarkers
Metabolic
Stoichiometry
Chromatography
Q-Index Code B1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Book Chapter
Collection: UQ Centre for Clinical Research Publications
 
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