Development of universal genetic markers based on single-copy orthologous (COSII) genes in Poaceae

Liu, Hailan, Guo, Xiaoqin, Wu, Jiasheng, Chen, Guo-Bo and Ying, Yeqing (2013) Development of universal genetic markers based on single-copy orthologous (COSII) genes in Poaceae. Plant Cell Reports, 32 3: 379-388. doi:10.1007/s00299-012-1371-4


Author Liu, Hailan
Guo, Xiaoqin
Wu, Jiasheng
Chen, Guo-Bo
Ying, Yeqing
Title Development of universal genetic markers based on single-copy orthologous (COSII) genes in Poaceae
Journal name Plant Cell Reports   Check publisher's open access policy
ISSN 1432-203X
0721-7714
Publication date 2013-03
Year available 2012
Sub-type Article (original research)
DOI 10.1007/s00299-012-1371-4
Volume 32
Issue 3
Start page 379
End page 388
Total pages 10
Place of publication Heidelberg, Germany
Publisher Springer
Collection year 2013
Language eng
Abstract Being evolutionary conserved, single-copy orthologous (COSII) genes are particularly useful in comparative mapping and phylogenetic investigation among species. In this study, we identified 2,684 COSII genes based on five sequenced Poaceae genomes including rice, maize, sorghum, foxtail millet, and brachypodium, and then developed 1,072 COSII markers whose transferability and polymorphism among five bamboo species were further evaluated with 46 pairs of randomly selected primers. 91.3 % of the 46 primers obtained clear amplification in at least one bamboo species, and 65.2 % of them produced polymorphism in more than one species. We also used 42 of them to construct the phylogeny for the five bamboo species, and it might reflect more precise evolutionary relationship than the one based on the vegetative morphology. The results indicated a promising prospect of applying these markers to the investigation of genetic diversity and the classification of Poaceae. To ease and facilitate access of the information of common interest to readers, a web-based database of the COSII markers is provided (http://www.sicau.edu.cn/web/yms/PCOSWeb/PCOS.html).
Keyword COSII genes
Poaceae
Polymorphism
Transferability
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online: 12 December 2012.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Brain Institute Publications
Official 2013 Collection
 
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Created: Tue, 12 Feb 2013, 16:30:05 EST by Debra McMurtrie on behalf of Queensland Brain Institute