A dominant mutation in mec-7/β-tubulin affects axon development and regeneration in Caenorhabditis elegans neurons

Kirszenblat, Leonie, Neumann, Brent, Coakley, Sean and Massimo Hilliard (2013) A dominant mutation in mec-7/β-tubulin affects axon development and regeneration in Caenorhabditis elegans neurons. Molecular Biology of the Cell, 24 3: 285-296. doi:10.1091/mbc.E12-06-0441


Author Kirszenblat, Leonie
Neumann, Brent
Coakley, Sean
Massimo Hilliard
Title A dominant mutation in mec-7/β-tubulin affects axon development and regeneration in Caenorhabditis elegans neurons
Formatted title
A dominant mutation in mec-7/β-tubulin affects axon development and regeneration in Caenorhabditis elegans neurons
Journal name Molecular Biology of the Cell   Check publisher's open access policy
ISSN 1059-1524
1939-4586
Publication date 2013-02-01
Year available 2012
Sub-type Article (original research)
DOI 10.1091/mbc.E12-06-0441
Open Access Status DOI
Volume 24
Issue 3
Start page 285
End page 296
Total pages 12
Place of publication Bethesda, MD, United States
Publisher American Society for Cell Biology
Collection year 2013
Language eng
Formatted abstract
Microtubules have been known for decades to be basic elements of the cytoskeleton. They form long, dynamic, rope-like structures within the cell that are essential for mitosis, maintenance of cell shape, and intracellular transport. More recently, in vitro studies have implicated microtubules as signaling molecules that, through changes in their stability, have the potential to trigger growth of axons and dendrites in developing neurons. In this study, we show that specific mutations in the Caenorhabditis elegans mec-7/β-tubulin gene cause ectopic axon formation in mechanosensory neurons in vivo. In mec-7 mutants, the ALM mechanosensory neuron forms a long ectopic neurite that extends posteriorly, a phenotype that can be mimicked in wild-type worms with a microtubule-stabilizing drug (paclitaxel), and suppressed by mutations in unc-33/CRMP2 and the kinesin-related gene, vab-8. Our results also reveal that these ectopic neurites contain RAB-3, a marker for presynaptic loci, suggesting that they have axon-like properties. Interestingly, in contrast with the excessive axonal growth observed during development, mec-7 mutants are inhibited in axonal regrowth and remodeling following axonal injury. Together our results suggest that MEC-7/β-tubulin integrity is necessary for the correct number of neurites a neuron generates in vivo and for the capacity of an axon to regenerate.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online before print December 5, 2012

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Brain Institute Publications
Official 2013 Collection
 
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Citation counts: TR Web of Science Citation Count  Cited 9 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 13 times in Scopus Article | Citations
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Created: Tue, 12 Feb 2013, 16:20:35 EST by Debra McMurtrie on behalf of Queensland Brain Institute