Short-term dose-response characteristics of 2-iminobiotin immediately post insult in the neonatal piglet following hypoxia-ischemia

Bjorkman, S. Tracey, Ireland, Zoe, Fan, Xiyong, van der Wal, Willem M., Roes, Kit C. B., Colditz, Paul B. and Peeters-Scholte, Cacha M. P. C. D. (2013) Short-term dose-response characteristics of 2-iminobiotin immediately post insult in the neonatal piglet following hypoxia-ischemia. Stroke, 44 3: 809-811+. doi:10.1161/STROKEAHA.112.677922


Author Bjorkman, S. Tracey
Ireland, Zoe
Fan, Xiyong
van der Wal, Willem M.
Roes, Kit C. B.
Colditz, Paul B.
Peeters-Scholte, Cacha M. P. C. D.
Title Short-term dose-response characteristics of 2-iminobiotin immediately post insult in the neonatal piglet following hypoxia-ischemia
Journal name Stroke   Check publisher's open access policy
ISSN 0039-2499
1524-4628
Publication date 2013-03
Sub-type Article (original research)
DOI 10.1161/STROKEAHA.112.677922
Open Access Status DOI
Volume 44
Issue 3
Start page 809
End page 811+
Total pages 12
Place of publication Philadelphia, PA, United States
Publisher Lippincott Williams & Wilkins
Collection year 2014
Language eng
Formatted abstract
Background and Purpose: To determine the optimal dose of 2-iminobiotin (2-IB) for the treatment of moderate to severe asphyxia in a neonatal piglet model of hypoxia-ischemia.

Methods: Newborn piglets were subjected to a 30-minute hypoxia-ischemia insult and randomly treated with vehicle or 2-IB (0.1 mg/kg, 0.2 mg/kg, or 1.0 mg/kg). aEEG background and seizure activity were scored after hypoxia-ischemia every 4 h until 24 h and at 48 h and neurobehavioral scores were obtained. Brain tissue was collected and processed for analysis of caspase-3 activity, histology, and tyrosine nitration.

Results: A dose range of 0.1 to 1.0 mg/kg/dose of 2-IB improved short-term outcome as demonstrated by an increased survival with a normal aEEG and decreased nitrotyrosine staining in the 2-IB-treated animals, indicating decreased cellular damage. Neurobehavior, caspase-3 activity in thalamus, and histology scores were not significantly different.

Conclusions: Based on survival with a normal aEEG, 0.2 mg/kg 2-IB is likely to be the most appropriate dose for use in future clinical trials in neonates with perinatal hypoxia-ischemia.
Keyword Hypoxia-ischemia, brain
2-iminobiotin
Neuroprotection
Neonatal nursing
Nitric oxide synthase
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2014 Collection
School of Medicine Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 4 times in Thomson Reuters Web of Science Article | Citations
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Created: Mon, 11 Feb 2013, 14:34:20 EST by Mrs Maureen Pollard on behalf of Paediatrics & Child Health - RBWH